人脐带间充质干细胞对大鼠子宫内膜异位症病灶神经纤维的影响

山东大学学报(医学版) ›› 2013, Vol. 51 ›› Issue (3): 48-51.

人脐带间充质干细胞对大鼠子宫内膜异位症病灶神经纤维的影响

陈岩1，李栋2，张哲1，时庆2，汪大琨2，刘欢2，王国云1

山东大学齐鲁医院 1.妇产科， 2.低温医学实验室，济南 250012

收稿日期:2012-12-22 出版日期:2013-03-10 发布日期:2013-03-10
通讯作者: 王国云(1971- )，女，博士，教授，硕士生导师，主要从事子宫内膜异位症的基础与临床研究。 E-mail: wangguoy@sdu.edu.cn
作者简介:陈岩(1986- )，女，硕士研究生，主要从事子宫内膜异位症的基础与临床研究。 E-mail:hncy1986@163.com
基金资助:
国家自然科学基金面上项目(81070469，81270671)；山东省自然科学基金(2009ZRB02130); 山东大学自由探索项目基金(2009TS070);教育部第43批留学回国人员启动基金(20111568)；济南市留学回国人员创业计划(20120205)

Effects of human umbilical cord mesenchymal stem cells on nerve fibers in the lesions of rat endometriosis model

CHEN Yan1, LI Dong2, ZHANG Zhe1, SHI Qing2, WANG Da-kun2, LIU Huan2, WANG Guo-yun1

1. Department of Obstetrics and Gynecology； 2. Cryomedicine Laboratory,
Qilu Hospital of Shandong University， Jinan 250012, China

Received:2012-12-22 Online:2013-03-10 Published:2013-03-10

摘要/Abstract

摘要：

目的探讨人脐带间充质干细胞(hUC-MSCs)对大鼠子宫内膜异位症(EMs)病灶神经纤维的影响。方法 hUC-MSCs从人新鲜脐带组织中分离和培养，并经过流式细胞仪鉴定。将体质量180~210g成年雌性Wistar大鼠，通过手术成功被诱导为EMs模型。将模型鼠随机分为治疗组和对照组，各12只。2周后治疗组接受含有hUC-MSCs的生理盐水尾静脉注射两周，1次/周，对照组接受相同体积和次数的生理盐水注射。4周后子宫内膜异位病灶被收集。标本被切片和用抗神经丝蛋白(NF)和神经生长相关蛋白-43(GAP-43)抗体进行免疫组化。结果与对照组相比，治疗组的NF平均光密度值明显低于对照组(P＜0.01)； GAP-43的平均光密度值与对照组相比也明显降低(P＜0.01)。结论 hUC-MSCs能够降低EMs病灶的神经纤维密度值。

关键词: 子宫内膜异位症；间充质干细胞；神经纤维；人脐带；大鼠

Abstract:

Objective To investigate the influence of human umbilical cord mesenchymal stem cells (hUC-MSCs) on nerve fibers in the lesions of rat models of endometriosis (EMs). Methods hUC-MSCs were isolated and cultured from fresh human umbilical cord, then identified by flow cytometry. Adult female Wistar rats, weighing 180-210g, were surgically induced EMs. Thereafter the EMs rat models were randomly divided into two groups (12 rats each), namely the treatment group and the control group. Two weeks later, the treatment group received hUCMSCs by injection of normal saline into the tail vein once a week for two weeks. Meanwhile, the control group only received the same volume of normal saline. EMs lesions were collected four weeks later. The specimens were sectioned and stained immunohistochemically with antibodies against Neurofilament (NF) and Growth Associated Protein 43 (GAP-43). Results Compared to that of the control group, the mean density of NF-immunoreactive nerve fibres of the treatment group was remarkably lower(P＜0.01). Besides, the mean density of nerve fibres stained with GAP-43 was also much lower in the treatment group in comparison with that of the control group(P＜ 0.01). Conclusion MSCs from human umbilical cord could reduce the nervefiber density of EMs lesions.

Key words: Endometriosis; Mesenchymal stem cells; Nerve fibers; Human umbilical cord; Rats

中图分类号:

R711.71

陈岩1，李栋2，张哲1，时庆2，汪大琨2，刘欢2，王国云1. 人脐带间充质干细胞对大鼠子宫内膜异位症病灶神经纤维的影响[J]. 山东大学学报(医学版), 2013, 51(3): 48-51.

CHEN Yan1, LI Dong2, ZHANG Zhe1, SHI Qing2, WANG Da-kun2, LIU Huan2, WANG Guo-yun1. Effects of human umbilical cord mesenchymal stem cells on nerve fibers in the lesions of rat endometriosis model[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(3): 48-51.

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