固相萃取-高效液相色谱法分析食用油中的苯并芘

山东大学学报(医学版) ›› 2012, Vol. 50 ›› Issue (8): 124-127.

固相萃取-高效液相色谱法分析食用油中的苯并芘

杨燕，付亚群，吴晓萌，姜青青，邵丽华

山东大学公共卫生学院卫生检验研究所, 济南 250012

收稿日期:2012-01-16 出版日期:2012-08-10 发布日期:2012-08-10
通讯作者: 邵丽华(1958- )，女，教授，主要从事色谱分析在卫生检验中的应用研究。 E-mail:slh@sdu.edu.cn
作者简介:杨燕(1987- )，女，硕士研究生，主要从事色谱分析在卫生检验中的应用研究

Determination of the Benzo(a)pyrene level in cooking oil by solid
phase extractionhigh performance liquid chromatography

YANG Yan, FU Ya-qun, WU Xiao-meng, JIANG Qing-qing, SHAO Li-hua

Institute of Health Laboratory Technology, School of Public Health， Shandong University, Jinan 250012， China

Received:2012-01-16 Online:2012-08-10 Published:2012-08-10

摘要/Abstract

摘要：

目的建立一种高效液相色谱-荧光检测器检测食用油中苯并芘的方法。方法对济南市场上食用油中苯并芘含量进行调查分析，采用SHIMADZU shimpack VPODS (250mm×4.6mm, 5μm)色谱柱，以甲醇∶水(90∶10，V/V)为流动相，流速为1.0mL/min，样品使用无键合硅胶固相萃取柱处理后，在激发波长285nm、发射波长406nm处进行检测。结果方法的最低检出限(S/N=3)为0.039μg/kg，样品的加标回收率为85.8%～90.7%，日内精密度为2.9%～4.1%，日间精密度为3.7%～5.3%。结论该方法灵敏度高，选择性好，定量准确，适用于食用油中苯并芘的检测。

关键词: 高效液相色谱法；荧光检测器；食用油；苯并芘；固相萃取

Abstract:

Objective To establish a high performance liquid chromatography with fluorescence detector for the determination of the benzo(a)pyrene(B(a)P) level in the cooking oil. Methods The difference of B(a)P content in edible oil in Jinan market was investigated by the SHIMADZU shim-pack VP-ODS (250mm×4.6mm, 5μm) column. The mobile phase was methanol-water(90∶10, V/V ), and the flow rate was 1. 0mL/min. The samples were treated by the non-bonded silica solid phase extraction(SPE) column, and then detected at the 285nm excitation wavelength and 406nm emission wavelength. Results The lowest limit of determination was 0.039μg/kg (S/N=3). The recovery rate was 85.8%-90.7%, and the intra-day and inter-day relative standard deviations were 2.9%-4.1% and 3.7%-5.3%. Conclusion This method is high sensitive, good selective and quantitative accurate, which is suitable for the B(a)P determination in the cooking oil.

Key words: High-performance liquid chromatography； Fluorescence detector； Cooking oil； Benzo(a)pyrene； Solid phase extraction

中图分类号:

R 115

杨燕，付亚群，吴晓萌，姜青青，邵丽华. 固相萃取-高效液相色谱法分析食用油中的苯并芘[J]. 山东大学学报(医学版), 2012, 50(8): 124-127.

YANG Yan, FU Ya-qun, WU Xiao-meng, JIANG Qing-qing, SHAO Li-hua. Determination of the Benzo(a)pyrene level in cooking oil by solid
phase extractionhigh performance liquid chromatography[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(8): 124-127.

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