关键词: 糖皮质激素受体；脾脏；1型单纯疱疹病毒；面瘫

Abstract:

Objective   To investigate expression of glucocorticoid receptor(GR) in the spleen of the mouse facial nerve palsy model induced by herpes simplex virus type 1. Metheods   Four-week-old male Balb/c mice were selected and randomly divided into three groups: the blank control group, the saline control group and the experimental group.HSV-1 was inoculated into the left auricle of the experimental mice by scratching with a 26-gauge needle, and the same volume of saline was placed in the left auricle of the controls. The spleens were obtained at 6h,12h, 24h, 2d, 3d, 5d and 7d after facial paralysis. Western blot and immunohistochemistry were used to detect the level of GR protein, while rea-time PCR  was used to measure the level of GR mRNA. Results   Compared with the saline control group, expressions of GR mRNA in the spleens of the experimental group  were slightly decreased after the facial paralysis, sharply declined at 24h, peaked on the 3rd day(all P<0.05), but returned to the baseline level on the 7th day(P>0.05). GR protein expression in the experimental group was similar to GR mRNA. Conclusions   Expression of GR in the spleen of the mouse facial nerve palsy model induced by herpes simplex virus type 1 appears in a time-dependent pattern which at first is reduced, then increases and finally returns to the baseline .It implies that GR expression in the spleen of the model follows a pattern, and may play a role in the process of the disease.        

Key words: Glucocorticoid  Receptor； Spleen; Herpes simplex virus type 1; Facial paralysis