Abstract:

Objective   To examine HMGA1 expression in glioblastoma cell line U251 and glioma stem cells (GSCs) isolated from it.  Methods   GSCs expressing the surface marker CD133 were isolated from U251 using an MACS column. Immunofluorescence and flow cytometry analyses were performed on GSCs. GSCs was compared with U251 in HMGA1 expression at the transcriptional (real-time PCR) and translational (Western blotting) levels. Results   GSCs were isolated from U251. The percentage of GSCs in U251 was about 0.32%. In GSCs, HMGA1 expression was 6.13±0.25 times higher at the transcriptional level and 2.75±0.99 times higher at the translational level than in U251. Conclusion   HMGA1 is overexpressed in GSCs as compared to U251, which implied that HMGA1 plays an important role in malignant proliferation, invasion, and differentiation of tumors in vivo. HMGA1 could be used as a biomarker and a  therapeutic target for glioblastoma and GSC.

Key words: Glioblastoma multiforme; Gliomastemcells; High mobility group A1