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山东大学学报(医学版) ›› 2010, Vol. 48 ›› Issue (4): 27-.

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芩丹胶囊对血管外膜成纤维细胞的作用机制

任敏1,张继东1,王博1,刘萍2,姜虹3,刘桂林1   

  1. 山东大学 1. 齐鲁医院中医科, 济南 250012; 2. 第二医院健康管理科, 济南 250033;
    3. 齐鲁医院心血管重构与功能实验室, 济南 250012
  • 收稿日期:2009-11-17 出版日期:2010-04-16 发布日期:2010-04-16
  • 通讯作者: 张继东,教授,博士生导师,主要研究方向为中西医结合心血管病。 Email:drzjd@sdu.edu.cn
  • 作者简介:任敏(1982- ),女,博士研究生,主要研究方向为中西医结合心血管病。
  • 基金资助:

    国家自然科学基金资助课题(30873324)

Effect of Qindan Capsule on arterial adventitial fibroblasts

REN Min1, ZHANG Jidong1, WANG Bo1, LIU Ping2, JIANG Hong3, LIU Guilin1   

  1. 1. Department of Traditional Chinese Medicine, Qilu Hospital of Shandong University, Jinan 250012, China;
    2. Department of Health Management, Second Hospital of Shandong University, Jinan 250033, China;
    3. Laboratory of Cardiovascular Remodeling and Function Research, Qilu Hospital of Shandong University, Jinan 250012, China
  • Received:2009-11-17 Online:2010-04-16 Published:2010-04-16

摘要:

目的  探讨芩丹胶囊(QC)含药血清对动脉血管外膜成纤维细胞(AF)的作用机制。方法  采用组织贴块法体外原代培养大鼠胸主动脉成纤维细胞,MTT法观察大[750mg/(kg·d)]、小[150mg/(kg·d)]剂量的QC含药血清对转化生长因子β1 (TGF-β1)诱导的大鼠胸主动脉成纤维细胞的增殖的影响;实时定量PCR及westernblot分别检测α-平滑肌肌动蛋白(α-SM actin)和Smad3的基因及蛋白表达情况。结果  大、小剂量QC含药血清呈剂量依赖性抑制TGF-β1诱导的AF的增殖(P<0.05),且用药组α-SM actin和Smad3基因及蛋白的表达较单纯刺激组显著降低(P<0.05)。结论  QC含药血清通过干扰TGF-β1/Smad信号转导通路抑制AF增殖和分化,从而改善和逆转动脉血管外膜重构。

关键词: 芩丹胶囊;转化生长因子β1/Smad;成纤维细胞;增殖;大鼠

Abstract:

Objective  To investigate the effect and mechanism of the Qindan Capsule-containing serum on adventitial fibroblasts(AFs). Methods  AFs were cultured by tissue explant in vitro and induced by TGF-β1. The proliferation of AFs affected by the Qindan Capsule-containing serum with high[750mg/(kg·d)] or low[150mg/(kg·d)] dose was detected by MTT. Protein and mRNA expressions of α-SM actin and Smad3 were observed by Western-blot and Real-time PCR respectively. Results  Both high and low doses of the Qindan Capsule-containing serum could reduce proliferation of AFs induced by TGF-β1(P<0.05) and inhibit expressions of mRNA and protein of α-SM actin and Smad3(P<0.05). Conclusion  The Qindan Capsule-containing serum has significant improved and reverse effect on aortic vascular remodeling. The mechanism may be related to inhibiting  proliferation and differentiation of AFs through the  TGF-β1/Smad signaling pathway.

Key words: Qindan Capsule; Transforming growth factor-beta1/Smad; Adventitial fibroblast; Proliferation; Rats

中图分类号: 

  • R543.5
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