乙醇对人肝L02细胞糖原和GSK3β、PAMPK的影响

山东大学学报(医学版)

乙醇对人肝L02细胞糖原和GSK3β、PAMPK的影响

于慧^1,2，陈少华¹，赵家军²，高聆³

（山东大学 1.附属千佛山医院内分泌科，济南 250014；
2. 附属省立医院内分泌科，济南 250021； 3. 附属省立医院中心实验室，济南 250021）

收稿日期:1900-01-01 修回日期:1900-01-01 发布日期:2009-04-16
通讯作者: 陈少华

Effects of ethanol on glycogen content, GSK3β，
and PAMPK of L02 cells

YU Hui^1,2, CHEN Shaohua¹, ZHAO Jiajun², GAO Ling³

(1. Department of Endocrinology, Qianfoshan Hospital Affiliated to Shandong University, Jinan 250014, China;
2. Department of Endocrinology, Provincial Hospital Affiliated to Shandong University, Jinan 250021, China;
3. Department Central Lab, Provincial Hospital Affiliated to Shandong University, Jinan 250021, China)

Received:1900-01-01 Revised:1900-01-01 Published:2009-04-16
Contact: CHEN Shaohua

摘要/Abstract

摘要： 目的研究乙醇对体外培养的人肝L02细胞糖原含量及糖原合成酶激酶(GSK3β)、磷酸化的AMP激活的蛋白激酶(PAMPK)的影响。方法培养L02细胞，按加入的乙醇浓度分为0、50和100?mmol/L三组，培养24?h，用蒽酮-硫酸比色法测定细胞糖原含量，蛋白免疫印记法(Western Blot)检测GSK3β及PAMPK的表达水平；并加入AMPK的激动剂(AICAR)或AMPK的阻断剂(Compound C)，检测GSK3β含量的变化。结果与0?mmol/L组比较，50和100?mmol/L组糖原含量下降、GSK3β及PAMPK表达增强(P均<0.05)，50?mmol/L组与100?mmol/L组比较，糖原含量及蛋白表达均未见明显统计学差异(P>0.05)；AICAR和Compound C对GSK3β的表达无明显影响(P>0.05)。结论乙醇能减少L02细胞糖原含量，可能与GSK3β表达增加有关。

关键词: 乙醇, 糖原, Ca(2+)钙调蛋白依赖性蛋白激酶, 蛋白激酶类

Abstract: To investigate the effects of ethanol on glycogen content, glycogen synthase kinase3β (GSK3β), and phosphoAMPactivated protein kinase (PAMPK) of human liver L02 cells in vitro. MethodsL02 cells were treated with ethanol and were divided into the 0, 50 or 100?mmol/L group. After treatment for 24 hours, all cells were collected and were further analyzed. The Glycogen content was determined by anthronesulfuric acid colorimetry, and expression of both GSK3β and PAMPK was observed by western blot analysis. After AICAR and Compound C which works as a selective agonist or inhibitor of AMPK respectively treated the cells for 1 h prior to ethanol, GSK3β expression was determined. ResultsCompared with the 0?mmol/L group, the glycogen content decreased, while protein expression of both GSK3β and PAMPK increased in the 50 and 100?mmol/L group (P<0.05). No differences was shown in the glycogen content and protein expression between the 50 and 100?mmol/L group (P＞0.05). No expression changes of GSK3β were observed after either AICAR or Compound C treatment. ConclusionGlycogen content of L02 cells is decreased after ethanol exposure, which may be due to GSK3β activity.

Key words: Ethanol, Glycogen, Ca(2+)Calmodulin dependent protein Kinase, Protein kinases

中图分类号:

R575.5

于慧1,2 ，陈少华1 ，赵家军2 ，高聆3
. 乙醇对人肝L02细胞糖原和GSK3β、PAMPK的影响[J]. 山东大学学报(医学版), 2009, 47(04): 75-78.

YU Hui^1,2, CHEN Shaohua¹, ZHAO Jiajun², GAO Ling³
. Effects of ethanol on glycogen content, GSK3β，
and PAMPK of L02 cells[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2009, 47(04): 75-78.

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