DT3染毒大鼠肺脏组织损伤与灌洗液LDH、TAOC、TP浓度变化的研究

山东大学学报(医学版)

DT3染毒大鼠肺脏组织损伤与灌洗液LDH、
TAOC、TP浓度变化的研究

张明亮¹,侯立安²,张志利¹

(1. 第二炮兵工程学院研究生管理大队一队，西安 710025；
2. 第二炮兵工程设计研究院总工办，北京 100011)

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-01-16 发布日期:2009-01-16

Lung tissue damage and level changes of LDH,TAOC and TP of lavage in rats exposed to DT3

ZHANG Mingliang¹,　HOU Lian², ZHANG Zhili¹

(1. Second Artillery Engineering College, Xi′an 7100251, China;
2. Engineering Design and Research Institute of Second Artillery Corps, Beijing 100011, China)

Received:1900-01-01 Revised:1900-01-01 Online:2009-01-16 Published:2009-01-16

摘要/Abstract

摘要： 目的探讨单推3（DT3）的肺脏毒性效应及其机制。方法气管滴注染毒建立染毒模型，对SPF Wistar雄性大鼠连续染毒14?d，采用酶联免疫吸附实验(ELISA)对染毒组大鼠支气管肺泡灌洗液中的乳酸脱氢酶(LDH)、总抗氧化力(TAOC)、总蛋白质(TP)进行检测。结果高剂量组大鼠支气管肺泡灌洗液中LDH浓度显著性升高，TAOC浓度显著性降低（P均＜0.05），TP浓度各实验组与对照组差异无统计学意义。单细胞凝胶电泳实验测定肺组织细胞损伤荧光镜下可观察到随染毒剂量的增加，尾部DNA含量与尾矩有增加的趋势。结论DT3可引起肺脏的炎性反应导致膜损伤，高剂量时，由自由基引起的氧化损伤表现明显。彗星实验无法排除其引起DNA损伤的可能性。

关键词: 毒性试验, 彗星试验, 支气管肺泡灌洗液, 单推3, 大鼠,Wistar

Abstract: DT3 which is widely used in the aerospace field as a closedcorrectposecontrol propellant, has become the focus of concern because of its safety, especially in respect to its toxicology. To explore DT3 on the toxicology of lungs and its mechanism. MethodsSPF Wistar male rats were given the drug by bronchial injection 14 consecutive days. ResultsThe levels of LDH, TAOC and TP in the bronchalveolar lavage were determined through ELISA, the level of LDH was significantly increased and TAOC significantly decreased (P＜0.05) at a high dose, while no statistical significance of the TP concentration was found in all groups. Also, tail DNA intensity and tail moment were increased in a dosedependent manner in lung tissue cells as determined by fluoroscope through single cell gel electrophoresis. ConclusionDT3 might result in membrane damage by producing lung inflammation. Oxidant damage is not a contributing factor in toxicity at a low dose. The possibility that DT3 induces DNA damage could not be ruled out.

Key words: Toxicity tests, Comet assay, Bronchoalvolar lavage fluid, DT3, Rats,Wistar

中图分类号:

R994

张明亮1 ,侯立安2 ,张志利1
. DT3染毒大鼠肺脏组织损伤与灌洗液LDH、
TAOC、TP浓度变化的研究[J]. 山东大学学报(医学版), 2009, 47(01): 27-29.

ZHANG Mingliang¹,　HOU Lian², ZHANG Zhili¹
. Lung tissue damage and level changes of LDH,TAOC and TP of lavage in rats exposed to DT3[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2009, 47(01): 27-29.

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