兔急性脊髓损伤后磁刺激运动诱发电位与病理变化的相关性

山东大学学报(医学版)

兔急性脊髓损伤后磁刺激运动诱发电位与病理变化的相关性

侯勇¹，聂林¹,吴琦²，邵军³，吕丽红³

山东大学 1. 齐鲁医院骨创科； 2. 医学院解剖学教研室；3. 医学院手术学教研室，济南 250012

收稿日期:2008-07-15 修回日期:1900-01-01 出版日期:2008-09-16 发布日期:2008-09-16
通讯作者: 聂林

Transcranial magnetic stimulation motor evoked potentials and pathologicchanges in rabbit spinal cord injury

HOU Yong¹, NIE Lin¹, WU Qi², SHAO Jun³, LV Li-ping³

1. Department of Orthopaedic, Qilu Hospital of Shan Dong University; 2. Department of anatomy; 3. Department of Surgery, School of Medicine, Shandong University, Jinan 250012, China

Received:2008-07-15 Revised:1900-01-01 Online:2008-09-16 Published:2008-09-16
Contact: NIE Lin

摘要/Abstract

摘要： 目的观察兔急性脊髓损伤后经颅磁刺激运动诱发电位（transcranial magnetic stimulation motor evoked pontentials，TMS-MEP）的变化规律，并与脊髓病理变化相比较，探讨其相关性。方法将日本大耳兔32只随机分为4组，每组8只。A组为对照组，B、C、D组为实验组（脊髓损伤组）。然后对每组动物分别于麻醉后、暴露脊髓后、伤后5min、30min、1h、6h、1d、3d、7d检测TMS-MEP，对所得波形数据进行统计学分析。于伤后1d、3d、7d采用后肢的Tarlov分级行运动功能评分。以损伤部位为中心切取脊髓标本，行HE染色光镜、电镜观察。结果随着脊髓损伤的加重，MEP的潜伏期逐渐延长，波幅在逐渐减小，病理显示白质纤维脱髓鞘越重，神经细胞损伤数目越多，MEP的潜伏期延长和波幅降低越明显。结论经颅磁刺激运动诱发电位与脊髓病理变化有明显的相关性，TMS-MEP对脊髓损伤十分敏感，能客观地检测脊髓运动功能，反映脊髓损伤程度。

关键词: 病理, 兔, 脊髓损伤, 运动诱发电位, 经颅磁刺激

Abstract: To explore the changes of transcranial magnetic stimulation motor evoked potentials(TMSMEP) and spinal cord pathology in rabbit spinal cord injury, and to explore their correlation. MethodsA digital weight drop apparatus induced spinal cord lesion was performed to mimic human SCI. Based on the drop height, 32 rabbits were randomly divided into four groups: the control, 2cm, 4cm and 6cm injured groups. TMSMEP was used to assess neurologic function. The MEP was recorded at 5min, 15min, 30min, 60min, 6h, 24h, 3d, 7d post-injury and before spinal cord injury in injury groups. Tarlov behaviour score was performed before and after surgery at 1, 3, 7 days. Light and electron microscope were used to observe the pathological changes. Results Spinal cord injury resulted in a gradual decrement in the peak latency and significant increment in the peak amplitude. Simultaneously, histological studies showed that the drop causes a ischemia lesion, which was found in human spinal cord injury. ConclusionTMSMEP has a significant association with spinal cord pathological changes and will be a noninvasive examination for spinal cord function.

Key words: Transcranial magnetic stimulation, Motor evoked potential, spinal cord injury, Pathology, Rabbits

中图分类号:

R322.81

侯勇,聂林,吴琦,邵军,吕丽红 . 兔急性脊髓损伤后磁刺激运动诱发电位与病理变化的相关性[J]. 山东大学学报(医学版), 2008, 46(9): 855-858.

HOU Yong,NIE Lin,WU Qi,SHAO Jun,LV Li-ping. Transcranial magnetic stimulation motor evoked potentials and pathologicchanges in rabbit spinal cord injury[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2008, 46(9): 855-858.

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