液相色谱质谱联用技术测定动物血浆和组织中莪术醇

山东大学学报(医学版)

液相色谱质谱联用技术测定动物血浆和组织中莪术醇

郝震峰，王本杰，张蕊，魏春敏，袁桂艳，郭瑞臣

山东大学齐鲁医院临床药理研究所，济南 250012

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-08-16 发布日期:2008-08-16
通讯作者: 郭瑞臣

Determination of curcumol in animal plasma and tissues by HPLC-MS/MS

HAO Zhen-feng, WANG Ben-jie, ZHANG Rui, WEI Chun-min, YUAN Gui-yan, GUO Rui-chen

Institute of Clinical Pharmacology, Qilu Hospital of Shandong University, Jinan 250012, China

Received:1900-01-01 Revised:1900-01-01 Online:2008-08-16 Published:2008-08-16
Contact: GUO Rui-chen

摘要/Abstract

摘要：

目的〓〖HTK〗建立HPLC-MS/MS法测定动物组织和血浆中莪术醇浓度。
〖HTW〗方法〓〖HTK〗以奥硝唑为内标物，DiamonsilTM色谱柱C18(150mm×4.6mm, 5μm)为固定相，流速1mL/min，采用多反应离子检测方式测定犬血浆和大鼠5种组织样品中莪术醇（m/z 254.3→219.3）和内标奥硝唑（m/z 220.3→128.2）的浓度。〖HTW〗结果〓〖HTK〗在动物血浆和组织匀浆中，莪术醇浓度与内标物峰面积之比呈良好的线性关系。〖HTW〗结论〓〖HTK〗所建立的HPLC-MS/MS方法操作简便快速，灵敏度高，专属性强，可用于莪术醇动物药代动力学和组织分布研究。

关键词: 组织分布, 液相色谱-质谱联用, 莪术醇, 莪术油

Abstract:

Objective〓〖WTBZ〗To develop high performance liquid chromatography coupled with the tandem mass spectrometry(HPLC-MS/MS) quantitative detection method for determination of curcumol in animal plasma and tissues. 〖WTHZ〗Methods〓〖WTBZ〗Dog plasma and rat tissue samples spiked with ornidazole as the internal standard were separated on a Diamonsil C18 column and eluted at a flow rate of 1mL/min, and the precursor/ product ion combinations of m/z 254.3→219.4 and m/z 220.3→128.1 were monitored to quantify curcumol by MRM. 〖WTHZ〗Results〓〖WTBZ〗A good linearity of plasma or tissue curcumol concentration and area ratio of curcumol to i.s was obtained. 〖WTHZ〗Conclusion〓〖WTBZ〗The developed method is rapid, highly sensitive and specific and could be used in animal curcumol pharmacokinetic and tissue distribution studies.

Key words: Zedoary turmeric oil, Curcumol, Tissue distribution, High performance liquid chromatography coupled with tandem mass spectrometry

中图分类号:

R927.2

郝震峰,王本杰,张蕊,魏春敏,袁桂艳,郭瑞臣. 液相色谱质谱联用技术测定动物血浆和组织中莪术醇
[J]. 山东大学学报(医学版), 2008, 46(8): 823-827.

HAO Zhen-feng,WANG Ben-jie,ZHANG Rui,WEI Chun-min,YUAN Gui-yan,GUO Rui-chen

. Determination of curcumol in animal plasma and tissues by HPLC-MS/MS[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2008, 46(8): 823-827.

参考文献

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