川芎嗪与氨基胍对实验性胰岛素抵抗大鼠糖代谢的影响

山东大学学报(医学版)

川芎嗪与氨基胍对实验性胰岛素抵抗大鼠糖代谢的影响

魏树珍¹,张永欢²,陈融²,李莉²,冯复利²,李瑞峰²

1. 济南市中心医院妇产科, 济南 250013;2. 山东大学医学院病理学与病理生理学研究所, 济南 250012

收稿日期:2007-11-06 修回日期:1900-01-01 出版日期:2008-03-16 发布日期:2008-03-16
通讯作者: 李瑞峰

Effect of Tetromethylpragine and Aminoguanidine on metabolism of glucose in insulin resistance rats

WEI Shu-zhen¹,ZHANG Yong-huan²,CHEN Rong²,LI Li²,FENG Fu-li²,LI Rui-feng²

1. Department of Genocology and Obestrics, Jinan Central Hospital;2. Department of Pathophysiology, School of Medicine, Shandong University

Received:2007-11-06 Revised:1900-01-01 Online:2008-03-16 Published:2008-03-16
Contact: LI Rui-feng

摘要/Abstract

摘要： 目的探讨川芎嗪与氨基胍对高糖胰岛素抵抗大鼠诱导型一氧化氮合酶(inducible nitric oxide synthase,iNOS)和糖代谢的影响。方法雄性Wistar 大鼠50只,随机分为正常对照组(NC)、高糖对照组(FC)、氨基胍组(AG)、川芎嗪组(TMP)、川芎嗪与氨基胍联合治疗组(AT),每组10只,均普通饲料喂养,高糖对照和各治疗组饮用12％果糖水复制高果糖胰岛素抵抗模型。摄入高糖3个月后,AG组给予氨基胍50mg／(kg·d)、TMP组给予川芎嗪40mg／(kg·d)、AT组给予川芎嗪40mg／(kg·d)与氨基胍50mg／(kg·d)灌胃治疗6个月。于实验前、实验中1、2、3、5、7、9个月末分别取尾静脉空腹血,测定血糖、血浆胰岛素、胰岛素敏感指数、血浆一氧化氮代谢物(NO-2)、外周血白细胞iNOS mRNA表达。结果FC组与NC组比较,造模2个月,血浆NO-2含量、外周血白细胞iNOS mRNA表达明显升高(P＜0.01),并持续保持在高水平上。造模3个月,动物血糖与血浆胰岛素水平升高,胰岛素敏感指数显著降低(P＜0.01);AT治疗4～6个月,与FC组比较,血糖浓度降低,胰岛素敏感指数升高(P＜0.05);血浆NO-2含量、外周血白细胞iNOS mRNA阳性表达率降低(P＜0.01)。结论川芎嗪与氨基胍联合应用有抑制iNOS mRNA表达和缓解胰岛素抵抗作用。

Abstract: To study the effects of tetromethylpragine and aminoguanidine for the metabolism of glucose and the expressions of inducible nitric oxide synthase mRNA(iNOS mRNA) in experimental insulin resistance rats. MethodsFifty male Wistar rats were randomly divided into the NC, FC, AG, TMP, and AT groups. The FC, AG, TMP and AT groups were fed with 12％ fructose, and the NC group was fed with tap water. After 3 months, the AG group was treated with 50mg／(kg·d) aminoguanidine, the TMP group with 40mg／(kg·d) tetromethylpragine, and the AT group with 40mg／(kg·d) tetromethylpragine and 50mg／(kg·d) aminoguanidine for 6 months. The levels of blood sugar, insulin, NO-2 and the expression of iNOS mRNA were determined before the experiment and at the end of the 1st, 2nd, 3rd, 5th, 7th and 9th month. ResultsThe level of blood NO-2(P＜0.01) and the expression of the iNOS mRNA (P＜0.01) were increased in the fructose control group compared with the control group and they remained at a high level till the end of 2 months′ fructose intake. The plasma insulin and the blood glucose were both increased, and the insulin sensitivity index was significantly decreased in the fructose control group compared with the control group(P＜0.01) at the end of 3 months′ fructose intake. The blood glucose was decreased(P＜0.05), the insulin sensitivity index was increased (P＜0.05), and the NO-2 and the iNOS mRNA were decreased(P＜0.01) at the end of 4 months′ treatment with tetromethylpragine and aminoguanidine. ConclusionTetromethylpragine and aminoguanidine can inhibit the expression of the iNOS mRNA and improve insulin resistance in experimental rats of fructoseinduced insulin resistance.

Key words: Fructose, Insulin resistance, Nitric oxide, iNOS mRNA, Rats, Wistar

中图分类号:

R363-21

魏树珍,张永欢,陈融,李莉,冯复利,李瑞峰 . 川芎嗪与氨基胍对实验性胰岛素抵抗大鼠糖代谢的影响[J]. 山东大学学报(医学版), 2008, 46(3): 249-253.

WEI Shu-zhen,ZHANG Yong-huan,CHEN Rong,LI Li,FENG Fu-li,LI Rui-feng. Effect of Tetromethylpragine and Aminoguanidine on metabolism of glucose in insulin resistance rats[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2008, 46(3): 249-253.

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