结核性和肺腺癌性胸腔积液中肺表面活性物质相关蛋白A、D的检测分析

山东大学学报(医学版)

结核性和肺腺癌性胸腔积液中肺表面活性物质相关蛋白A、D的检测分析

崔纪云¹，田广燕²,林殿杰¹，龙成凤¹，初奎胜³

1. 山东大学附属省立医院呼吸内科, 济南 250021；2. 山东大学齐鲁儿童医院神经内科，济南 250022；3. 青岛大学附属医院海阳分院呼吸内科，山东海阳 265100

收稿日期:2007-11-08 修回日期:1900-01-01 出版日期:2008-02-16 发布日期:2008-02-16
通讯作者: 林殿杰

Detection of lung surfactant proteinA and D in pleural effusions due to lung adenocarcinoma and tubercular pleurisy

CUI Ji-yun¹，TIAN Guang-yan²,LIN Dian-jie¹，LONG Cheng-feng¹，CHU Kui-sheng³

1. Department of Respiratory Diseases, Provincial Hospital Affiliated to Shandong University, Jinan 250021, China;2. Department of Neurology, Qilu Child Hospital Affiliated to Shandong University

Received:2007-11-08 Revised:1900-01-01 Online:2008-02-16 Published:2008-02-16
Contact: LIN Dian-jie

摘要/Abstract

摘要： 目的探讨胸水中肺表面活性物质相关蛋白－A、D联合对原发性肺腺癌性与结核性胸腔积液的鉴别诊断价值。方法收集36例原发性肺腺癌性和28例结核性胸腔积液患者的胸水，采用 western blotting检测胸水中肺表面活性物质相关蛋白-A、D。结果原发性肺腺癌和结核性胸膜炎患者胸水中SPA光密度值分别为265.1±141.16和180.0±64.1；原发性肺腺癌和结核性胸膜炎患者胸水中SP-D光密度值分别为299.9±140.6和206.8±86.8。36例原发性肺腺癌患者中有23例胸水中SP-A＞250，有16例SP-D＞250，而SP-A＞250和（或）SP-D＞250有29例。28例结核性胸膜炎患者胸水中SP-A、SP-D均未超过250。结论肺表面活性物质相关蛋白A对结核性胸膜炎与原发性肺腺癌所致胸腔积液鉴别诊断有临床价值,联合肺表面活性物质相关蛋白D对两者的鉴别诊断更有意义。

Abstract: To study the value of combined determinations of surfactant -associated protein-D(SP-D) and -A (SP-A) in the pleural fluid in distinguishing lung adenocarcinoma and tubercular pleurisy. Methods SP-A and SP-D were determined in the pleural effusions from 36 patients with lung adenocarcinoma and 28 with tubercular pleurisy by western blotting. Results SP-A concentrations in pleural effusions due to lung adenocarcinoma and tubercular pleurisy were 265.1±141.16 and 180.0±64.1 respectively, and SP-D concentrations were 299.9±140.6 and 206.8±86.8, respectively. SP-A＞250 was found in 23 of 36 lung adenocarcinomas，SP-D＞250 in 16 of 36 lung adenocarcinomas， while increased values of SP-A and/or SP-D were found in pleural effusions from 29 of 36 lung adenocarcinomas. SP-A and -D values did not exceed 250 in any of 28 patients with tubercular pleurisy. Conclusions SP-A is a useful immunohistochemical marker for lung adenocarcinoma, and SP-A in pleural effusion is valuable in diagnosis of primary lung adenocarcinoma and tubercular pleurisy, however, a combination of SP-D and -A assays in pleural effusions will be better for discriminating lung adenocarcinoma from tubercular pleurisy.

Key words: Pleural effusion, Tuberculosis, pleura, Lung neoplasms, SP-A, SP-D

中图分类号:

R561.4

崔纪云,田广燕,林殿杰,龙成凤,初奎胜 . 结核性和肺腺癌性胸腔积液中肺表面活性物质相关蛋白A、D的检测分析[J]. 山东大学学报(医学版), 2008, 46(2): 196-199.

CUI Ji-yun,TIAN Guang-yan,LIN Dian-jie,LONG Cheng-feng,CHU Kui-sheng. Detection of lung surfactant proteinA and D in pleural effusions due to lung adenocarcinoma and tubercular pleurisy[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2008, 46(2): 196-199.

参考文献

多维度评价

Viewed

Full text

Abstract

Cited

Shared

Discussed