复方水蛭素的药效学研究

山东大学学报(医学版)

复方水蛭素的药效学研究

王杰¹，韩俊庆²，李伯辉³，任青华⁴，张博⁴，李焱⁴，真钢⁴，姜枫勤⁴

1. 山东省职业卫生与职业病防治研究院，山东济南 250002；2. 山东省立医院肿瘤中心，山东济南 250021；3. 山东省医学科学院附属医院，山东济南 250062；4. 山东省医学科学院基础所，山东济南 250062

收稿日期:2007-06-10 修回日期:1900-01-01 出版日期:2007-08-24 发布日期:2007-08-24
通讯作者: 韩俊庆

Pharmacodynamics of compound hirudin

WANG Jie¹，HAN Jun－qing²，LI BO－hui³，REN Qing－hua⁴，ZHANG Bo⁴，LI Yan⁴，ZHEN Gang⁴，JIANG Feng－qin⁴

1. Shangdong Academy of Occupational Health and Occupational Medicine；2. Tumor Research and Treatment Center, Shandong Provincial Hospital；3. Affiliated Hospital of Shandong Academy of Medical Sciences

Received:2007-06-10 Revised:1900-01-01 Online:2007-08-24 Published:2007-08-24
Contact: HAN Jun-qing

摘要/Abstract

摘要： 目的：探讨复方水蛭素抗肿瘤的作用机理。方法：①对W256肿瘤的影响：于大白鼠左前腋下接种W256瘤种细胞液(0.3ml/只)，次日开始用药。阴性对照组给予生理盐水；阳性对照组给予5-FU；中药组分别给予不同剂量的复方水蛭素；②对胃癌靶细胞（NKM）的影响：将药物稀释成1∶10、1∶20、1∶40、1∶80、1∶160、1∶320、1∶640的浓度，接种于多孔培养板内的人胚胎肺细胞(HEL)和肿瘤靶细胞(NKM)上，37℃、CO₂培养，观察细胞病变，计算抑瘤指数；③对超氧化物歧化酶（SOD）和脂质过氧化物（MDA）的影响：取小鼠44只，随机分成四组，给药方法同W256肿瘤实验组，连续用药30d，次日摘取小鼠眼球取血，用微量快速检测法测定SOD和MDA的含量。结果：复方水蛭素对W256肿瘤具有明显的抑制作用（P<0.01），该药浓度在1∶80（最大抑瘤浓度）时抑制肿瘤靶细胞的效应最大，1∶640（最小抑瘤浓度）时，抑制肿瘤靶细胞的效应最小。同时，复方水蛭素还能明显提高小鼠血中SOD的活性和降低MDA代谢产物的含量（P<0.05）。结论：复方水蛭素具有明显的体内外抗肿瘤作用。

Abstract: Objective: To investigate the anti-tumor mechanism of hirudin. Methods: ①W256 tumors were transplanted into the left armpit of rats. Then they were divided into three groups: the negative control group was subjected to saline, the positive control group was subjected to 5-Fu, and the traditional Chinese medicine group was subjected to compound hirudin. ② The drug was diluted to a ratio of 1∶10，1∶20，1∶40，1∶80, 1∶160, 1∶320, and 1∶640 and was implanted into a porous plate for HEL and NKM under a 37℃ and CO2 condition. The inhibition effect was determined. ③ 40 mice were also divided into groups as the first experiment. 30 days later, the SOD and MDA contents were determined in the eyeball blood by a trace rapid assay. Results: Compound hirudin effectively inhibited the growth of W256 tumors（P<0.01）and was the most significant at a ratio of 1:80 and was the least significant at a ratio of 1:640. It also can significantly increase SOD activity and reduce MDA metabolity（P<0.05）. Conclusion:Compound hirudin can significantly affect the W256 tumor in vivo, the NKM in vitro and the blood SOD and MDA.

Key words: Compound hirudin, W256 tumor, Superoxide dismutase, Lipid peroxidation

中图分类号:

R917

王杰,韩俊庆,李伯辉,任青华,张博,李焱,真钢,姜枫勤 . 复方水蛭素的药效学研究[J]. 山东大学学报(医学版), 2007, 45(8): 852-854.

WANG Jie,HAN Jun－qing,LI BO－hui,REN Qing－hua,ZHANG Bo,LI Yan,ZHEN Gang,JIANG Feng－qin. Pharmacodynamics of compound hirudin[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2007, 45(8): 852-854.

参考文献

相关文章 9

[1]	钟丽红1，赵宇2，刘德山3. 益气补肾中药脑神康胶囊的质量控制标准研究[J]. 山东大学学报(医学版), 2010, 48(10): 116-119.
[2]	周慧1,于宗琴2,李军3,李刚1,纪洪生1. 三种镇静催眠药对阿司匹林酯酶活性的影响[J]. 山东大学学报(医学版), 2010, 48(4): 151-.
[3]	黄萍凌霄. HPLC/MS/MS法测定抗风湿中药制剂中糖皮质激素类化学药[J]. 山东大学学报(医学版), 2009, 47(12): 138-140.
[4]	高秀蕊臧恒昌. 复方烟酸辛伐他汀双层片体外释放度的研究[J]. 山东大学学报(医学版), 2209, 47(6): 109-113.
[5]	祝保林, 高锦红, 李吉峰 . 普卢利沙星的吸附伏安特性及其应用[J]. 山东大学学报(医学版), 2009, 47(01): 98-102.
[6]	徐洁,袁桂艳,魏春敏,王本杰,郭瑞臣 . HPLC测定人尿液三苯双脒次级代谢物对苯二甲酸浓度[J]. 山东大学学报(医学版), 2008, 46(10): 1016-1019.
[7]	武静,王本杰,魏春敏,孔祥麟,郭瑞臣. HPLC法测定丹皮酚血浓度及其胶囊与片剂人体生物等效性研究[J]. 山东大学学报(医学版), 2007, 45(7): 726-729.
[8]	高秀蕊臧恒昌. 复方烟酸辛伐他汀双层片体外释放度的研究[J]. 山东大学学报(医学版), 2009, 47(6): 109-.
[9]	. 稳心胶囊的制备及质量标准[J]. 山东大学学报(医学版), 2009, 47(7): 136-140.

多维度评价

Viewed

Full text

Abstract

Cited

Shared

Discussed