大鼠动脉粥样硬化形成过程中TAFI水平变化及作用机制

山东大学学报(医学版)

大鼠动脉粥样硬化形成过程中TAFI水平变化及作用机制

刘利妍，刘向群，陈效安，陈焕芹

山东大学齐鲁医院保健科，山东济南 250012

收稿日期:2006-12-26 修回日期:1900-01-01 出版日期:2007-08-24 发布日期:2007-08-24
通讯作者: 刘向群

TAFI level and its mechanism in the process of atherosclerosis in rats

LIU Li-yan, LIU Xiang-qun, CHEN Xiao-an, CHEN Huan-qin

Department of Geriatrics, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China

Received:2006-12-26 Revised:1900-01-01 Online:2007-08-24 Published:2007-08-24
Contact: LIU Xiang-qun

摘要/Abstract

摘要： 目的：探讨在大鼠动脉粥样硬化（AS）形成过程中凝血酶活化的纤维蛋白溶解抑制剂（TAFI）活性水平变化及作用机制。方法：将40只健康的雄性Wistar大鼠随机分为正常对照组、高脂组、高脂维生素D3组和内皮损伤组，每组10只。分别以普通饲料、高脂饲料、高脂饲料+维生素D3负荷、高脂饲料+维生素D3负荷+内皮球囊损伤处理4个月，复制大鼠动脉粥样硬化形成过程中三个阶段的模型，即高脂血症、纤维增生性动脉硬化、较成熟的AS斑块病变。模型建造前，自颈动脉取血，模型建造成功后，自下腔静脉取血，用全自动生化仪测定血浆总胆固醇（TC）、总甘油三脂（TG）、低密度脂蛋白（LDL-C）、高密度脂蛋白（HDL-C），发色底物法测定血浆TAFI的活性，分析TAFI活性以及血脂指标在造模前、后不同组间的变化。结果：① 成功复制了大鼠动脉粥样硬化发展过程中高脂血症期、纤维增生性动脉硬化期、较成熟的斑块期的模型；② 高脂组、高脂维生素D3组和内皮损伤组大鼠的TC、TG、LDL-C水平和TAFI活性明显高于正常对照组（P＜0.01），且有递增趋势，以内膜损伤组升高最为明显；三组模型鼠的HDL-C水平明显低于正常对照组（P＜0.01或P＜0.05），且有递减趋势，以内膜损伤组降低最为明显；③ 血浆TAFI活性与TC和TG水平呈正相关。结论：AS过程中,TAFI水平呈逐渐增高趋势，提示AS的病变程度与血浆TAFI 水平密切相关。

Abstract: Objective: To investigate the level of TAFI and its mechanism on atherosclerosis. Methods: Forty healthy male Wistar rats were randomly divided into 4 groups (n=10): the control group, the high lipid group, the high lipid + Vitamin D overload group, and the endothelium injury group. Then the content of TC, TG, HDL-C, and LDL-C and the activity of TAFI were determined by a chromogenic assay. Results: Atherosclerosis was successfully established. Hyperlipoidemia stage, fiber hyperplasy arteriosclerosis stage and maturatly plaque stage were also successfully duplicated. TC, TG, LDL-C and the activity of TAFI in the plasma of the three model groups gradually increased compared with the control group （P＜0.01). However, HDL-C in the plasma of the three model groups gradually decreased compared with the control group（P＜0.01 or P＜0.05). The activity of TAFI in the plasma positively related to TG and TC. Conclusion: The activity of TAFI in the plasma is closely correlated with atherosclerosis severity.

Key words: Atherosclerosis, Thrombin activatable fibrinolysis inhibitor, Endothelium, Injuries, Rats, Wistar

中图分类号:

R541.4

刘利妍,刘向群,陈效安,陈焕芹. 大鼠动脉粥样硬化形成过程中TAFI水平变化及作用机制[J]. 山东大学学报(医学版), 2007, 45(8): 787-791.

LIU Li-yan,LIU Xiang-qun,CHEN Xiao-an,CHEN Huan-qin. TAFI level and its mechanism in the process of atherosclerosis in rats[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2007, 45(8): 787-791.

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