醒脑静注射液对急性脑缺血大鼠炎症反应及病理的影响

山东大学学报(医学版)

醒脑静注射液对急性脑缺血大鼠炎症反应及病理的影响

吴玉生¹，张文高²，郑国玲¹，张剑¹

1. 山东大学西区医院内科，山东济南 250012；2. 山东中医药大学基础医学院内科教学研究中心，山东济南 250014

收稿日期:2006-11-06 修回日期:1900-01-01 出版日期:2007-07-24 发布日期:2007-07-24
通讯作者: 张文高

Effect of Xingnaojing injection on inflammatory reaction and brain edema and preparations on rats with acute brain ischemia

WU Yu-sheng¹，ZHANG Wen-gao²，ZHENG Guo-ling¹，ZHANG Jian¹

１. Department of Internal Medicine, Western Hospital of Shandong University,Jinan 250012, Shandong, China； 2． Medical Teaching Research Center，Shandong University of Traditional Chinese Medicine

Received:2006-11-06 Revised:1900-01-01 Online:2007-07-24 Published:2007-07-24
Contact: ZHANG Wen-gao

摘要/Abstract

摘要： 目的：探讨醒脑静注射液对大鼠急性脑缺血损伤的保护作用。方法：75只大鼠随机分为模型组、对照组、醒脑静组，每组25只，模型组与对照组腹腔注射生理盐水(4ml/Kg·体重),醒脑静组腹腔注射醒脑静注射液(4ml/Kg·体重)，均为每天2次，共5d。然后对模型组及醒脑静组大鼠采用结扎双侧颈总动脉法造成急性脑缺血模型，术后药物剂量加倍注射1次。观察三组大鼠血清肿瘤坏死因子a（TNF-α）、细胞间粘附分子-1（ICAM-1）水平、脑含水量、脑组织病理形态。结果：模型组大鼠血清TNF-α、ICAM-1水平、脑含水量显著高于醒脑静组和对照组（P均＜0.01）；光镜下观察大鼠脑组织病理形态可见，模型组损伤最明显，醒脑静组次之，对照组损伤不明显。相关分析表明，血清TNF-α水平与ICAM-1水平呈正相关性（P＜0.01）；血清TNF-α、ICAM-1水平与脑含水量呈正相关性（P＜0.01）。结论：醒脑静注射液可抑制炎症反应、减轻脑水肿程度、减轻病理损伤，提示醒脑静注射液对脑缺血有一定的保护作用。

Abstract: Objective: To explore the protection of Xingnaojing injection(XI) on rats with acute brain ischemia. Methods： Seventy-five rats were randomly divided into three groups( 25 in each). The control and model groups were subjected to 0.9% Nacl(4ml/Kg)and the XI group was subjected to XI (4ml/Kg) through the abdominal cavity, twice a day for 5 days. Then the two sides of the general carotids of the model and XI groups were tied-up to cause acute brain ischemia. After the operations, they were subjected to double dosages. Serum tumor necrosis factor-a(TNF-α), intercellular adhension molecule-1(ICAM1) levels, brain edema and preparations were determined. Results： Serum TNF-α, ICAM-1 levels and brain edema of the model group were higher than those of the control and XI groups(P＜0.01). The injury degree of preparations of the model group was heavier than that of the XI group. There were positive correlations between TNF-α and ICAM-1（P＜0.01）, between TNF-α and brain edema(P＜0.01),and between ICAM1 and brain edema（P＜0.01. Conclusion： XI could inhibit the inflammatory reaction and decrease brain edema, and protect the acute brain ischemia of rats.

Key words: Xingnaojing injection, Brain ischemia, Tumor necrosis factor-a, Intercellular adhension molecule-1, Brain edema, Preparations, Rats, Wistar

中图分类号:

R743.3

吴玉生,张文高,郑国玲,张剑 . 醒脑静注射液对急性脑缺血大鼠炎症反应及病理的影响[J]. 山东大学学报(医学版), 2007, 45(7): 746-748.

WU Yu-sheng,ZHANG Wen-gao,ZHENG Guo-ling,ZHANG Jian. Effect of Xingnaojing injection on inflammatory reaction and brain edema and preparations on rats with acute brain ischemia[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2007, 45(7): 746-748.

参考文献

多维度评价

Viewed

Full text

Abstract

Cited

Shared

Discussed