猪胶原基真皮支架成纤维细胞生长特性的观察

山东大学学报(医学版)

猪胶原基真皮支架成纤维细胞生长特性的观察

张爱君¹,宋国栋¹,曹成波²，汪运山³，卞东会¹

1. 山东大学临床医学院济南中心医院烧伤整形科，山东济南 250013；2. 山东大学化学与化工学院，山东济南 250100；3. 山东大学临床医学院济南中心医院中心实验室，山东济南 250013

收稿日期:2006-11-21 修回日期:1900-01-01 出版日期:2007-07-24 发布日期:2007-07-24
通讯作者: 宋国栋

An experimental study on the attachment and growth of human skin fibroblast on a porcine collagenic dermal matrix in vitro

ZHANG Ai-jun¹,SONG Guo-dong¹, CAO Cheng-bo²，WANG Yun-shan³，BIAN Dong-hui¹

1. Department of Burn and Plastic Surgery；2. School of Chemistry and Chemical Engineering,Shandong University；3. Central Laboratory, Jinan Central Hospital, School of Clinical Medicine, Shandong University

Received:2006-11-21 Revised:1900-01-01 Online:2007-07-24 Published:2007-07-24
Contact: SONG Guo-dong

摘要/Abstract

摘要： 目的：观察成纤维细胞在一种新型的猪胶原基真皮支架（PCDM）上的粘附生长特性，探讨提高异种真皮支架细胞相容性的方法。方法:分别在PCDM乳头层面和网状层面种植人皮肤成纤维细胞，于接种后1、7、14d通过HE染色，扫描和透射电镜下观察成纤维细胞在PCDM上的生长情况。结果:人皮肤成纤维细胞早期在乳头层真皮面的粘附优于网状真皮面，而晚期更多的网状真皮面可见到细胞形成复层并长入基质内部。结论:PCDM具有良好的细胞相容性，是与异体真皮最为接近的天然真皮支架，改良生产工艺以及去除基底膜和真皮乳头层可提高其细胞相容性。

Abstract: Objective: To observe the attachment and growth of fibroblasts on a new porcine collagenic dermal matrix（PCDM） and investigate a method to improve the cell compatibility of the porcine dermal scaffold. Method: The fibroblasts were seeded on two sides of the PCDM. 1, 7 and 14 days later， the composites were respectively observed by HE staining, scanning electron microscope（SEM）and transmission electron microscope（TEM）. Results: Fibroblasts attached better on the papillary dermis than on the reticular dermis in the early period, but more samples of the reticular dermis demonstrated fibroblast stratified structure and infiltration below the cellseeded surface than the papillary dermis. Conclusion: The new PCDM has good compatibility with human fibroblasts in vitro. It is the most similar to human skin. Modification of the processing technology and removal of the papillary dermis is capable of improving the cell compatibility of the PCDM.

Key words: Cell compatibility, Fibroblast, Dermal scaffold

中图分类号:

R622.1

张爱君,宋国栋,曹成波,汪运山,卞东会 . 猪胶原基真皮支架成纤维细胞生长特性的观察[J]. 山东大学学报(医学版), 2007, 45(7): 718-721.

ZHANG Ai-jun,SONG Guo-dong,CAO Cheng-bo,WANG Yun-shan,BIAN Dong-hui. An experimental study on the attachment and growth of human skin fibroblast on a porcine collagenic dermal matrix in vitro[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2007, 45(7): 718-721.

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