COX-2的表达与直肠腺癌淋巴管生成及淋巴结转移的关系

山东大学学报(医学版)

COX-2的表达与直肠腺癌淋巴管生成及淋巴结转移的关系

王敬源^1,2，付勤烨²，丁印鲁²，唐思锋^1,2，张建良²

山东大学 1. 医学院，山东济南 250012； 2. 第二医院普通外科，山东济南 250033

收稿日期:2006-11-06 修回日期:1900-01-01 出版日期:2007-07-24 发布日期:2007-07-24
通讯作者: 张建良

COX-2 expression in rectal adenocarcinoma and its relationgship with lymphangiogenesis and lymph node metastasis

WANG Jing-yuan^1,2，FU Qin-ye²，DING Yin-lu²，LIANG Si-feng^1,2，ZHANG Jian-liang²

1.School of Medicine, Shandong University；2.Department of General Surgery, Second Hospital of Shandong University

Received:2006-11-06 Revised:1900-01-01 Online:2007-07-24 Published:2007-07-24
Contact: ZHANG Jian-liang

摘要/Abstract

摘要： 目的：探讨环氧化酶-2(cyclooxygenase-2，COX-2)与血管内皮生长因子C(vascular endothelial growth factor C，VEGF-C)在直肠腺癌中的表达和意义及其相关性。方法：免疫组织化学PV-8000二步染色法，检测40例直肠腺癌原发灶标本和40例正常直肠黏膜中COX-2与VEGF-C蛋白的表达情况，并用血管内皮生长因子受体-3 (vascular endothelial growth factor receptor，VEGFR-3)作为淋巴管内皮细胞的特异性标记物进行免疫组化染色，观察肿瘤组织淋巴管生成状况。结果：所测40例直肠腺癌组织中，COX-2及VEGF-C的表达阳性率分别为62.5%(25／40)和65.0%(26／40)。40例标本中的21例（52.5%）可以检测到VEGFR3阳性的肿瘤新生淋巴管。COX-2表达与VEGF-C表达、肿瘤新生淋巴管形成及淋巴结转移、是否侵透浆膜层等临床病理指标间呈明显相关关系（P＜0.05）；但与肿瘤大小、肿瘤部位、病理组织学分型等无明显相关(P＞0.05)。结论：直肠腺癌组织中COX-2表达与VEGF-C表达状态、肿瘤淋巴管生成和淋巴结转移均呈明显相关；COX-2可能通过上调VEGF-C表达促进直肠腺癌淋巴管生成，并顺次导致淋巴结转移的发生。

Abstract: Objective: To investigate the expression of cyclooxygenase-2 (COX-2) and vascular endothelial growth factor-C (VEGF-C) in human rectal adenocarcinoma． Methods: COX-2 and VEGF-C expressions were determined in 40 cases of rectal adenocarcinoma and 40 cases of normal rectal mucous membrane by the immunohistochemical PV-8000 two-step staining method． Lymphanigogenesis was evaluated by immunohistochemical staining with the specific antibody, VEGFR-3． Results: Expression rate of COX-2 and VEGF-C was 62.5%(25／40) and 65.0%(26／40)， respectively in 40 rectal adenocarcinoma specimens． VEGFR3 was positive in 21 cases（21/40， 52.5%）indicating lymphangiogenesis in the tumors．Expression of COX-2 was significantly correlated with that of VEGF-C， tumor lymphangiogenesis and lymphatic metastasis and serosa invasion（P＜0.05)， however, it did not correlate with the tumor size， location and histological typing（P＜0.05）． Conclusions: In rectal adenocarcinoma， expression of COX-2 is significantly associated with VEGF-C， lymphangiogenesis and lymphatic metastasis． COX-2 may up-regulate the expression of VEGF-C， which induces lymphangiogenesis and contributes to lymphatic metastasis．

Key words: Rectal neoplasms, Cyclooxygenase-2, Lymphangiogenesis, Lymph node metastasis

中图分类号:

R735.37

王敬源,付勤烨,丁印鲁,唐思锋,张建良 . COX-2的表达与直肠腺癌淋巴管生成及淋巴结转移的关系[J]. 山东大学学报(医学版), 2007, 45(7): 711-713.

WANG Jing-yuan,FU Qin-ye,DING Yin-lu,LIANG Si-feng,ZHANG Jian-liang. COX-2 expression in rectal adenocarcinoma and its relationgship with lymphangiogenesis and lymph node metastasis[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2007, 45(7): 711-713.

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