胰岛素抵抗大鼠硫化氢/胱硫醚γ-裂解酶体系的实验观察

山东大学学报(医学版)

胰岛素抵抗大鼠硫化氢/胱硫醚γ-裂解酶体系的实验观察

于婷¹，侯为开¹，冯进波²，许聿新¹，董庆玉¹，张莉¹，张雯雯¹，陈丽¹

1. 山东大学齐鲁医院内分泌科，山东济南 250012；2. 教育部和卫生部心血管重构与功能研究重点实验室，山东济南 250012

收稿日期:2006-12-03 修回日期:1900-01-01 出版日期:2007-05-24 发布日期:2007-05-24
通讯作者: 侯为开

Changes of the hydrogen sulfide/cystathionine γ-lyase system in rats with insulin resistance

YU Ting¹，HOU Wei-kai¹，FENG Jin-bo²，XU Yu-xin¹，DONG Qing-yu¹，ZHANG Li¹，ZHANG Wen-wen¹，CHEN Li¹

1. Department of Endocrinology, Qilu Hospital of Shandong University；2. Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and of Public Health, Jinan 250012, Shandong, China

Received:2006-12-03 Revised:1900-01-01 Online:2007-05-24 Published:2007-05-24
Contact: HOU Wei-kai

摘要/Abstract

摘要： 目的：探讨硫化氢/胱硫醚γ-裂解酶(H2S/CSE)体系在胰岛素抵抗大鼠体内的变化,与高血压和胰岛素抵抗状态的关系。方法:随机将雄性健康Wistar大鼠30只分为正常对照组(NC组)、胰岛素抵抗对照组(IR组)、胰岛素抵抗加外源性H2S供体NaHS组(NaHS组)，每组各10只。IR组及NaHS组给予高糖饲料喂养6周建立代谢综合征模型，NC组给予普通饲料喂养。成模后NaHS组每日腹腔注射NaHS(56μmol／kg)，IR组及NC组腹腔注射同等剂量生理盐水，继续喂养6周后，检测其体重、血压、血脂、血糖、血胰岛素，血浆H2S水平和胸主动脉CSE mRNA转录水平, 计算胰岛素敏感指数。结果：IR组及NaHS组体重、血脂、血压、血胰岛素显著高于NC组(P＜0.01), 胰岛素敏感指数显著低于NC组(P＜0.01), CSE mRNA 转录水平及H2S水平均低于NC组大鼠(P＜0.01)。NaHS组CSE mRNA转录水平(P＜0.05)及H2S水平(P＜0.01)均高于IR组, 血压显著低于IR对照组(P＜0.01)，但体重、血脂、血压、血糖、血胰岛素、胰岛素敏感指数与IR组无显著差异。结论：胰岛素抵抗大鼠硫化氢/胱硫醚γ-裂解酶体系受到抑制，是其高血压形成的重要因素，外源性给予H2S有助于缓解高血压，但对于胰岛素敏感性无明显改善作用。

关键词: 胸, , 主动脉, 硫化氢, 胰岛素抵抗, 高血压

Abstract: Objective: To explore the changes of hydrogen sulfide/cystathionine γ-lyase(H2S/CSE) system in rats with insulin resistance and the effect of the H2S/CSE system on the development of hypertension and insulin resistance. Methods: Thirty male Wistar rats were randomly divided into three groups: the normal control(NC) group, the insulin resistant(IR) group and the NaHS(H2S donor)-treated(NaHS) group. The insulin resistant rat model was established by a highglucose diet of 6 weeks. Rats in the NaHS group were subjected to NaHS(56μmol／kg) and rats in the other two groups were subjected to normal saline every day. Six weeks later, the body weight(BW), systolic blood pressure(SBP), fasting blood sugar(FBG), fasting serum insulin(FSI), cholesterin(TC), triglyceride(TG), the transcriptional level of CSE in aorta and the H2S level in plasma were measured, and the insulin sensitive index(ISI) was calculated. Results: Compared with the NC group, BW, SBP, FSI, TC, TG in the other two groups significantly increased (P＜0.01), and the ISI, transcriptional levels of CSE and the H2S levels decreased (P＜0.01). Exogenous administration of NaHS made the transcriptional levels of CSE (P＜0.05) and the H2S levels (P＜0.01) more significantly increased in the NaHS group than in the IR group. Compared with the IR group, SBP in the NaSH group significantly decreased (P＜0.01), but BW, FBG, FSI, TC, TG and ISI did not change a lot. Conclusion: The H2S/CSE system is downregulated in rats with insulin resistance and is a major factor in the development of hypertension. Exogenous administration of H2S may attenuate the process of hypertension, but seems not to affect the insulin sensitive index.

Key words: Insulin resistance, Hypertension, Hydrogen sulfide, Aorta thoracic

中图分类号:

Q95-33

于婷,侯为开,冯进波,许聿新,董庆玉,张莉,张雯雯,陈丽 . 胰岛素抵抗大鼠硫化氢/胱硫醚γ-裂解酶体系的实验观察[J]. 山东大学学报(医学版), 2007, 45(5): 490-494.

YU Ting,HOU Wei-kai,FENG Jin-bo,XU Yu-xin,DONG Qing-yu,ZHANG Li,ZHANG Wen-wen,CHEN Li. Changes of the hydrogen sulfide/cystathionine γ-lyase system in rats with insulin resistance[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2007, 45(5): 490-494.

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