全反式维甲酸对体外培养的人Tenon囊成纤维细胞增殖及凋亡的影响

山东大学学报(医学版)

全反式维甲酸对体外培养的人Tenon囊成纤维细胞增殖及凋亡的影响

戴丽华，李维宁，乔智

山东大学齐鲁医院眼科，山东济南 250012

收稿日期:2007-01-28 修回日期:1900-01-01 出版日期:2007-04-24 发布日期:2007-04-24
通讯作者: 乔智

Effect of all trans-retinoic acid on the proliferation and apoptosis of human Tenon's capsule fibroblasts in vitro

DAI Li-hua, LI Wei-ning, QIAO Zhi

Department of Ophthalmology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China

Received:2007-01-28 Revised:1900-01-01 Online:2007-04-24 Published:2007-04-24
Contact: QIAO Zhi

摘要/Abstract

摘要： 目的：观察全反式维甲酸(ATRA)对体外培养的人Tenon囊成纤维细胞（HTCFs）增殖及凋亡的影响。方法：体外培养HTCFs，采用流式细胞仪检测细胞周期改变、细胞凋亡率、抗凋亡基因bcl-2的水平，琼脂糖凝胶电泳检测细胞DNA，研究不同浓度的ATRA对体外培养的HTCFs增殖及凋亡的影响，同时与丝裂霉素C（MMC）作比较。结果：用ATRA和MMC处理后，HTCFs细胞周期的分布发生了明显的变化，表现为G⁰/G¹期细胞数增多，S期细胞数减少；1×10_-9mol/L的ATRA即可诱导细胞的凋亡，随着浓度的增加，凋亡率显著增高；ATRA组bcl-2的水平比空白对照组大约低了一倍；DNA琼脂糖凝胶电泳结果显示：ATRA组为呈一定间隔的DNA梯形条带，MMC组呈现弥漫的片状图谱，无明显的条带。结论：ATRA可有效抑制体外培养的HTCFs的增殖，但与MMC的作用方式不同。MMC主要造成细胞坏死，而ATRA则主要诱导细胞凋亡，无明显细胞毒作用。

Abstract: Objective: To study the effect of all trans-retinoic acid (ATRA) on the proliferation and apoptosis of human Tenon′s capsule fibroblasts(HTCFs) in vitro. Methods: Cell cycle distribution, the apoptotic rate and the expression of bcl-2 were determined by flow cytometry. Cell DNA was determined by agarose gel electrophoresis. Mitomycin C was used as a positive control. Results: Cell cycle distribution of HTCFs showed that cells of the G⁰/G¹ phase increased and of the S phase decreased after the treatment with ATRA and MMC. Cellular apoptosis was decreased by ATRA at a lower dose (1×10_-9mol/L), and the apoptotic rate was significantly increased in a dose-dependent manner. Expression of bcl-2 was significantly decreased by ATRA. A “ladder” strand of DNA was produced of the ATRA groups by DNA agarose gel electrophoresis. Conclusion: ATRA can significantly inhibit the proliferation of HTCFs and induce the apoptosis of HTCFs without apparent cytotoxicity.

Key words: Retinoic acid, Fibroblasts, Tenon′s capsule, Cell proliferation, Apoptosis

中图分类号:

R775.5

戴丽华,李维宁,乔智. 全反式维甲酸对体外培养的人Tenon囊成纤维细胞增殖及凋亡的影响[J]. 山东大学学报(医学版), 2007, 45(4): 376-379.

DAI Li-hua,LI Wei-ning,QIAO Zhi. Effect of all trans-retinoic acid on the proliferation and apoptosis of human Tenon's capsule fibroblasts in vitro[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2007, 45(4): 376-379.

参考文献

多维度评价

Viewed

Full text

Abstract

Cited

Shared

Discussed