不同碘浓度对培养血管内皮细胞增殖的影响

山东大学学报(医学版)

不同碘浓度对培养血管内皮细胞增殖的影响

王晓磊^1，2，徐丽雅³，张海涛¹，李加美¹，郭成浩¹

1. 山东大学医学院，山东济南 250012； 2. 济南市中级人民法院，山东济南 250001；3. 济南钢铁集团职工医院，山东济南 250101

收稿日期:2007-01-17 修回日期:1900-01-01 出版日期:2007-03-24 发布日期:2007-03-24
通讯作者: 郭成浩

Effects on endothelial cell proliferation induced by different concentration of iodine in vitro

WANG Xiao-lei^1，2，XU Li-ya³，ZHANG Hai-tao¹，LI Jia-mei¹，GUO Cheng-hao¹

1. School of Medicine, Shandong University, Jinan 250012, Shandong， China；2.The Intermediate Court of Jinan City, Jinan 250001

Received:2007-01-17 Revised:1900-01-01 Online:2007-03-24 Published:2007-03-24
Contact: GUO Cheng-hao

摘要/Abstract

摘要： 目的：探讨不同碘浓度对人脐静脉血管内皮细胞增殖的影响。方法:体外培养人脐静脉血管内皮细胞株HUECV-304，在培养液中加入不同浓度的碘化钾，共分9组，分别为正常对照组及加碘组（0.5、1.0、2.0、3.0、4.0、5.0、6.0、8.0μmol/L），连续培养24h后，观察细胞形态改变并细胞计数，MTT比色法检测细胞活性。结果:0.5～4.0μmol/L剂量组在24h内内皮细胞计数和MTT检测均明显高于对照组，且细胞形态没有异常。而5.0μmol/L以上组，虽在8h内内皮细胞数目和MTT检测均明显高于对照组，但12h后内皮细胞数目和MTT检测均明显低于对照组；显微镜观察在4h始即可看见内皮细胞内有中毒颗粒出现，且随浓度增加和时间延长而增加，并出现明显的内皮形态变化。结论:碘对体外培养的内皮细胞有刺激增殖的作用，其作用与投碘的剂量及作用时间有关。

Abstract: Objective： To research the effects on proliferation of human umbilical vein endothelial cells (HUVECs) induced by different doses of iodine. Methods： HUVECs were subjected to iodine in various concentrations (0.5, 1.0, 2.0, 3.0, 4.0, 5.0, 6.0, and 8.0μmol/L)for 24 hours, and the morphology of the cultured endothelium was observed. MTT colorimetry was used to determine the proliferation activities of the cultured endothelium. Results： Iodine significantly increased the number and MTT activities of vascular endothelial cells from 0.5μmol/L to 4.0μmol/L compared with the normal group at 24 hours. Cell morphology was normal under a microscope. The number and MTT activities of vascular endothelial cells were significantly increased by iodine at over 5.0μmol/L at 8 hours, but were decreased at 12 hours and later. Poison granules in the endothelium were produced by iodine at over 5.0μmol/L at 4 hours and increased in a dose-and time-manner. Conclusion： Iodine effects on the proliferation activity of endothelial cells are dose-and time-dependent.

Key words: Iodine, Human umbilical vein endothelial cells, Cell culture, Proliferation

中图分类号:

R363.16

王晓磊,徐丽雅,张海涛,李加美,郭成浩 . 不同碘浓度对培养血管内皮细胞增殖的影响[J]. 山东大学学报(医学版), 2007, 45(3): 310-312.

WANG Xiao-lei,XU Li-ya,ZHANG Hai-tao,LI Jia-mei,GUO Cheng-hao. Effects on endothelial cell proliferation induced by different concentration of iodine in vitro[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2007, 45(3): 310-312.

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