蝎毒多肽提取物对肿瘤生长和细胞免疫功能的影响

山东大学学报(医学版)

蝎毒多肽提取物对肿瘤生长和细胞免疫功能的影响

张维东，张月英，王朝霞，王兆朋，贾青

山东省医学科学院基础医学研究所，山东现代医用药物与技术重点实验室，山东济南 250062

收稿日期:2007-01-10 修回日期:1900-01-01 出版日期:2007-03-24 发布日期:2007-03-24
通讯作者: 张维东

Effect of polypeptide extract from scorption venom on tumor growth and cellular immunity in rats with W256 sarcocarcinoma

ZHANG Wei-dong, ZHANG Yue-ying, WANG Zhao-xia, WANG Zhao-peng, JIA Qin

Institute of Basic Medicine, Shandong Academy of Medical Sciences, Key Laboratory of Modern Medicine and Technology of Shandong Province， Jinan 250062, Shandong, China

Received:2007-01-10 Revised:1900-01-01 Online:2007-03-24 Published:2007-03-24
Contact: ZHANG Wei-dong

摘要/Abstract

摘要： 目的：观察蝎毒多肽提取物（PESV）对大鼠W256癌肉瘤抑制作用及其对荷瘤大鼠细胞免疫功能的影响。方法：将30只Wistar荷瘤大鼠，随机分为模型组、5-FU治疗组和蝎毒治疗组，连续灌胃给蝎毒多肽提取物7d，处死，计算各组抑瘤率；流式细胞术（FCM）检测外周血T淋巴细胞亚群变化；酶联免疫吸附试验（ELISA）法测定血清中细胞因子INF-γ和IL-4含量。结果：蝎毒治疗组抑瘤率为55.21%。与模型组相比较，蝎毒治疗组荷瘤大鼠外周血中CD4+T细胞比例明显提高，CD8+T细胞明显降低，CD4+T/CD8+T值明显增高，差异均具有统计学意义（P＜0.05）；血清中细胞因子INF-γ含量明显升高，而IL-4含量显著降低，差异均具有统计学意义（P＜0.05）。结论：蝎毒多肽提取物对W256荷瘤大鼠肿瘤有明显抑制作用，通过提高细胞免疫功能，逆转荷瘤机体Th1/Th2漂移，改变荷瘤机体免疫抑制状态，发挥抗肿瘤作用。

Abstract: Objective: To study the inhibitory mechanism of polypeptide extract from scorpion venom (PESV) on W256 sarcocarcinoma and its influence on T subsets in rats. Methods: Tumor models were established by subcutaneously implanting W256 tumor cells into thirty Wistar rats. The tumor-bearing rats were randomly divided into three groups: the model group, the 5-fluorouracil(5-FU) group, and the PESV group. PESV was intragastrically subjected to the rats of the experimental group for 7 days. Then, the tumor-bearing rats were killed, and T subsets in the peripheral blood were determined by flow cytometry (FCM). The presence of interferon-gamma (INF-γ) and interleukin-4 (IL-4) were screened by an enzyme-linked immunosorbent assay (ELISA). Results： PESV had an inhibitory effect on tumor growth, and the growth inhibitory rate was 55.2%. The number of CD4+T and the CD4+T/CD8+T ratio of W256-bearing rats treated with PESV significantly increased compared with the model group (P＜0.05). The expression level of interferon-gamma (INF-γ) was up-regulated and of interleukin-4 (IL-4) down-regulated. Compared with the control group, the difference was significant(P＜0.05). Conclusion: PESV has an inhibitory effect on tumor growth by strengthening the immunocellular function. It could be regarded as a candidate of tumor inhibition.

Key words: Scorpion venom, Immunocompetence, CD4+T/CD8+T, Th1/Th2 shift

中图分类号:

R979.1

张维东,张月英,王朝霞,王兆朋,贾青. 蝎毒多肽提取物对肿瘤生长和细胞免疫功能的影响[J]. 山东大学学报(医学版), 2007, 45(3): 286-289.

ZHANG Wei-dong,ZHANG Yue-ying,WANG Zhao-xia,WANG Zhao-peng,JIA Qin. Effect of polypeptide extract from scorption venom on tumor growth and cellular immunity in rats with W256 sarcocarcinoma[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2007, 45(3): 286-289.

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