bFGF和TNF-α在颈椎后纵韧带骨化中的作用

山东大学学报(医学版)

bFGF和TNF-α在颈椎后纵韧带骨化中的作用

王修文，谯勇，吴东进

山东大学第二医院骨科，山东济南 250033

收稿日期:2006-07-24 修回日期:1900-01-01 出版日期:2007-03-24 发布日期:2007-03-24
通讯作者: 王修文

Expression of bFGF and TNF-α in the ossification of the posterior longitudinal ligament of the cervical spine

WANG Xiu-wen， QIAO Yong， WU Dong-jin

Department of Orthopaedics, Second Hospital of Shandong University, Jinan 250033, Shandong, China

Received:2006-07-24 Revised:1900-01-01 Online:2007-03-24 Published:2007-03-24
Contact: WANG Xiu-wen

摘要/Abstract

摘要： 目的：探讨碱性成纤维细胞生长因子(bFGF)和肿瘤坏死因子(TNF-α)在颈椎后纵韧带中的表达水平及在骨化发病机制中的作用。方法：采用免疫组织化学法，对30例骨化颈椎后纵韧带标本进行bFGF和TNF-α检测，与40例正常颈椎后纵韧带标本进行对照，再利用SPSS10.0行FISHER精确检验，分析有无统计学意义。结果：正常对照组bFGF表达10例阳性，30例阴性，阳性率25%；颈椎后纵韧带骨化组bFGF表达24例阳性，6例阴性，阳性率80%，差异有统计学意义(P＜0.05)。正常对照组TNF-α表达32例阳性，8例阴性，阳性率80%；颈椎后纵韧带骨化组TNF-α表达10例阳性，20例阴性，阳性率33.3%，差异有统计学意义(P＜0.05)。结论：bFGF的异常高表达和TNF-α的低表达促进了颈椎后纵韧带骨化的发生，这两种因子的异常表达在该病发病机制中有重要作用。

Abstract: Objective: To investigate the expression and function of bFGF and TNF-α in the ossification of the posterior longitudinal ligament(OPLL) of the cervical spine. Methods: Thirty specimens and 40 normal controls were used in this study. The immunohistochemical method (SABC) was applied to determine the expression levels of bFGF and TNF-α. SPSS(version 10.0) software was used to analyze the data. Results: Ten specimens of normal posterior longitudinal ligaments were positive for bFGF (25%) and twenty-four specimens with COPLL were positive for bFGF (80%). Thirty-two specimens of normal posterior longitudinal ligaments were positive for TNF-α(80%) and ten specimens with COPLL were positive for TNF-α (33.3%). There was a significant difference (P＜0.05). Conclusions: The expression of bFGF in patients with COPLL is higher than that in normal posterior longitudinal ligaments(P＜0.05). High expression of bFGF and low expression of TNF-α play an important role in the pathogenesis of COPLL.

Key words: Longitudinal ligaments, Fibroblast growth factor 2, Tumor necrosis factor

中图分类号:

R681.5+3

王修文,谯勇,吴东进. bFGF和TNF-α在颈椎后纵韧带骨化中的作用[J]. 山东大学学报(医学版), 2007, 45(3): 279-282.

WANG Xiu-wen,QIAO Yong,WU Dong-jin. Expression of bFGF and TNF-α in the ossification of the posterior longitudinal ligament of the cervical spine[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2007, 45(3): 279-282.

参考文献

多维度评价

Viewed

Full text

Abstract

Cited

Shared

Discussed