二脱氧肌苷对背根神经节神经元突起生长的影响

山东大学学报(医学版)

二脱氧肌苷对背根神经节神经元突起生长的影响

刘花香¹，刘真²，杨向东³，王丽红²，李振中²

山东大学 1. 齐鲁医院风湿科； 2. 医学院解剖学教研室； 3. 齐鲁医院肾内科，济南 250012

收稿日期:2007-03-22 修回日期:1900-01-01 出版日期:2007-12-24 发布日期:2007-12-24
通讯作者: 李振中

Effects of didanosine on the outgrowth of dissociated cultured dorsal root ganglion neuronal

LIU Hua-xiang¹，LIU Zhen²，YANG Xiang-dong³，WANG Li-hong²，LI Zhen-zhong²

1. Department of Rheumatology, Qilu Hospital of Shandong University;2. Department of Anatomy, School of Medicine, Shandong University；3. Department of Nephrology, Qilu Hospital of Shandong University

Received:2007-03-22 Revised:1900-01-01 Online:2007-12-24 Published:2007-12-24
Contact: LI Zhen-zhong

摘要/Abstract

摘要： 目的探讨二脱氧肌苷（didanosine, ddI）对分散培养的胎鼠背根神经节（dorsal root ganglion, DRG）神经元胞体和突起生长的影响。方法分散培养的胎鼠DRG神经元用不同浓度的ddI (1μg/mL, 5μg/mL, 10μg/mL, 20μg/mL) 孵育，对分散培养的DRG神经元用微管相关蛋白2（microtubule associated protein 2, MAP2）免疫荧光标记后，用共聚焦激光扫描显微镜（confocal laser scanning microscope, CLSM）观察神经元胞体和突起的改变。结果分散培养的胎鼠DRG神经元用ddI孵育3d后，神经元突起的数目减少和长度变短，而神经元的胞体的大小则没有明显变化。结论ddI可直接影响分散培养的胎鼠DRG神经元突起的生长。

关键词: 共聚焦激光扫描显微镜 , 脊, 神经元, 神经节, 二脱氧肌苷

Abstract: Objective To investigate the effects of didanosine (ddI) on the outgrowth of dissociated cultured dorsal root ganglion (DRG) neuronal. MethodsDissociated cultured DRG cells of the rat embryos were treated with ddI at different concentrations (1μg/mL, 5μg/mL, 10μg/mL and 20μg/mL, respectively). After 3 days exposure to ddI, the dissociated DRG cells were processed by microtubule associated protein 2 (MAP2) immunofluorescent labeling and the changes of neuronal cell body and neurites were observed by confocal laser scanning microscopy (CLSM). ResultsThe number of neurites in each neuron was decreased and the length of neurites in each neuron was shortened after 3 days treatment with ddI as compared with those in the control group. Whereas alterations of the diameter of neuronal cell bodies were not observed compared with those in the control group. ConclusionddI directly effects the outgrowth of neurites of the DRG neurons but dose not cause neuronal cell body shrinking in dissociated DRG cell cultures.

Key words: Didanosine, Ganglion, spinal, Neurons, Confocal laser scanning microscope

中图分类号:

R322.85

刘花香,刘真,杨向东,王丽红,李振中 . 二脱氧肌苷对背根神经节神经元突起生长的影响[J]. 山东大学学报(医学版), 2007, 45(12): 1275-1277.

LIU Hua-xiang,LIU Zhen,YANG Xiang-dong,WANG Li-hong,LI Zhen-zhong. Effects of didanosine on the outgrowth of dissociated cultured dorsal root ganglion neuronal[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2007, 45(12): 1275-1277.

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