成人喉乳头状瘤组织中HPV/E7、ki-67表达与微血管密度的相关性

山东大学学报(医学版)

成人喉乳头状瘤组织中HPV/E7、ki-67表达与微血管密度的相关性

赵秋良,李海燕

山东大学医学院，山东济南 250012

收稿日期:2007-02-14 修回日期:1900-01-01 出版日期:2007-10-24 发布日期:2007-10-24
通讯作者: 李海燕

Relationship between the expression of HPV/E7,ki-67 and The microvessel dentisy in laryngeal papilloma in adults

ZHAO Qiu-liang, LI Hai-yan

School of Medicine, Shandong University, Jinan 250012, Shandong, China

Received:2007-02-14 Revised:1900-01-01 Online:2007-10-24 Published:2007-10-24
Contact: LI Hai-yan

摘要/Abstract

摘要： 目的：探讨人乳头状瘤病毒、细胞增殖活性及新生血管的生成在喉乳头状瘤中的作用。方法:选取21例成人喉乳头状瘤组织标本，以14例声带息肉和14喉鳞癌组织标本为对照组,PCR方法检测HPV16/E7的表达,免疫组化法检测Ki67、CD34的表达。结果:HPV16/E7在声带息肉、成人喉乳头状瘤和喉鳞癌组织中的阳性表达率分别为:14.3%、66.7%、85.7%；Ki-67标记指数（labeling index,LI）分别为6.30±2.03、13.73±3.42、25.55±3.92；MVD分别为3.97±1.38、14.61±1.64、25.34±2.63；差异有统计学意义(P＜0.01)。HPV16/E7, CD34和Ki67呈正相关（r＝0.854,P＜0.01;r=0.878,P＜0.01)。结论:Ki-67、MVD与细胞的增殖活化程度明显相关，可以作为反映成人喉乳头状瘤恶变潜能的指标之一。

关键词: 增殖细胞抗原, CD34 , 喉乳头状瘤, 喉肿瘤

Abstract: Objective: To explore the role of HPV16/E7, the cellular proliferation activity and the tumor angiogenesis in laryngeal papilloma(LP) in adults. Methods: Twenty tissues of LP in adults were selected in the experimental group, and 14 tissues of vocal cord polyp (VCP) and 14 tissues of laryngeal squamous cell carcinoma(LSCC) were enrolled in the control groups. Expression of HPV16/E7 was determined by the polymerase chain reaction(PCR) method, and of Ki-67 and CD34 by the immuneohistochemical SP method. Results: The expression rate of HPV16/E7 in VCP, LP and LSCC was 14.3%, 66.7%, and 85.7%, respectively. The labeling index(LI) of Ki-67 was 6.30±2.03, 13.73±3.42, and 25.55±3.92, respectively. The number of MVD was 3.97±1.38,14.61±1.64, and 25.34±2.63, respectively. The difference was significant (P＜0.01). The level of HPV16/E7, CD34 was positively correlated with that of Ki-67 in the tissues（r=0.854,P＜0.01; r=0.878,P＜0.01）. Conclusion: Ki-67 and MVD highly correlates with the tumor angiogenesis, cellular proliferation activity and pathological types, and they can be served as the malignant indicators for LP in adults.

Key words: Laryngeal neoplasms, Laryngeal papilloma, Proliferating cell antigen, CD34

中图分类号:

R739.65

赵秋良,李海燕. 成人喉乳头状瘤组织中HPV/E7、ki-67表达与微血管密度的相关性[J]. 山东大学学报(医学版), 2007, 45(10): 1030-1033.

ZHAO Qiu-liang,LI Hai-yan. Relationship between the expression of HPV/E7,ki-67 and The microvessel dentisy in laryngeal papilloma in adults[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2007, 45(10): 1030-1033.

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