IL-1促进子宫内膜异位症在位基质细胞增殖作用的研究

山东大学学报(医学版)

IL-1促进子宫内膜异位症在位基质细胞增殖作用的研究

张贵宇¹,任淑文²,张友忠¹,马道新³

1. 山东大学齐鲁医院妇产科，山东济南 250012； 2. 青岛市第八人民医院妇产科，山东青岛 266000；3.山东大学齐鲁医院分子生物学实验室，山东济南 250012

收稿日期:2005-10-29 修回日期:1900-01-01 出版日期:2006-09-24 发布日期:2006-09-24
通讯作者: 张贵宇

〖KH+9.2mmD]Proliferation of endometrial stromal cells in endometriosis induced by Interleukin1beta

ZHANG Gui-yu, REN Shu-wen,ZHANG You-zhong,MA Dao-Xin

1. Department of Obstetrics and Gynecology, Qilu Hospital of Shandong University, Jinan 250012,Shandong, China; 2. The 8th People′s Hospital of Qingdao, Qingdao 266000, Shandong, China;

Received:2005-10-29 Revised:1900-01-01 Online:2006-09-24 Published:2006-09-24

摘要/Abstract

摘要： 目的：研究白细胞介素1β（interlukin1β,L1β）对子宫内膜异位症（endometriosis,EMs）在位内膜基质细胞生长的影响，探讨IL1β在EMs发病中的作用。方法：培养EMs在位内膜基质细胞作为研究EMs的体外实验模型，加入不同浓度的IL1β后采用四唑盐比色法（MTT）观察细胞增殖情况、流式细胞计量仪（flow cytometry,FCM）检测细胞生长周期。结果：依据MTT结果所绘得的细胞生长曲线及FCM检测的细胞周期结果显示：IL1β作用后细胞生长旺盛，G0/G1期细胞所占比例明显低于G2/M期及S期，当IL1β的浓度为 1.0?ng/ml且作用12?h此作用最为显著。结论：IL1β可促进EMs在位内膜基质细胞由G0/G1期进入G2/M期及S期，对子宫内膜细胞生长具有促增殖作用。

Abstract: To investigate the proliferation of endometrial stromal cells in endometriosis by interleukin1beta(IL1β). Methods: The eurotopic endometrial stromal cells of endometriosis cultured within 3～5 passages were treated with IL1β in a dosage of 0 to 10.0?ng/ml, and then the cell proliferation and the cell cycle were measured by MTT and flow cytometry (FCM) respectively. Results: The endometrial stromal cells had been growing luxuriantly, and G0/G1 phase cells were few than G2/M and S phase cells. Moreover, the proliferation was the most prominent when the cells were incubated in the presence of IL1β at 1.0?ng/ml concentration for 12 hours. Conclusions: IL1β induces the proliferation of endometrial stromal cells of endometriosis and promotes the transformation of G0/G1 phase cells into G2/M and S phase.

Key words: Endometriosis, Interleukin1, Cell cycle

张贵宇,任淑文,张友忠,马道新 . IL-1促进子宫内膜异位症在位基质细胞增殖作用的研究[J]. 山东大学学报(医学版), 2006, 44(9): 932-934.

ZHANG Gui-yu,REN Shu-wen,ZHANG You-zhong,MA Dao-Xin. 〖KH+9.2mmD]Proliferation of endometrial stromal cells in endometriosis induced by Interleukin1beta[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2006, 44(9): 932-934.

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