不同强度和时程应激对大鼠海马HSP70mRNA习惯化表达的影响

山东大学学报(医学版)

不同强度和时程应激对大鼠海马HSP70mRNA习惯化表达的影响

邵淑红¹,李尊岭²,江虹³,吴江⁴,潘芳³

1．滨州医学院心理学教研室, 山东滨州256603； 2.滨州医学院生物化学与分子生物学教研室, 山东烟台 264003； 3. 山东大学医学院医学心理学研究所，山东济南 250012；4.山东中医药大学生理学教研室，山东济南 250014

收稿日期:2005-09-14 修回日期:1900-01-01 出版日期:2006-08-24 发布日期:2006-08-24
通讯作者: 邵淑红

Stress in different intensity and duration effects the habitual expression of HSP70mRNA on the hippocampus of rats

SHAO Shu-hong, LI Zun-ling, JIANG Hong,WU Jiang，PAN Fang

1.Department of Medical Psychology, Binzhou Medical College, Binzhou 256603, Shandong, China;2.Department of Biochemistry and Molecular Bioloy, Binzhou Medical College, Yantai 264003, Shandong, China;

Received:2005-09-14 Revised:1900-01-01 Online:2006-08-24 Published:2006-08-24

摘要/Abstract

摘要： 目的：观察重复低强度强迫游泳应激后大鼠海马HSP70mRNA表达及习惯化表达；应激强度改变对HSP70mRNA表达的影响。方法：采用反转录-聚合酶链反应（RTPCR）方法，观察重复强迫温水游泳应激导致大鼠海马HSP70mRNA表达的变化和不同应激强度对HSP70mRNA表达的影响。结果：重复强迫温水游泳可导致HSP70mRNA表达习惯化，当再给予4?℃冰水游泳应激时，可引起HSP70mRNA表达增加。结论：重复应激可以导致HSP70mRNA表达习惯化，高强度应激可以逆转低强度应激引起的习惯化效应。

Abstract: To study the habitual expression of HSP70mRNA in hippocampus of rats by forcedswimming stress, and therefore to explore the effect of different intensity stress on the HSP70mRNA expression. Method: RTPCR was used to observe the changes of HSP70mRNA expression in hippocampus. Result: Repeated forced swimming in warm water resulted in expressive habituation, and an additive forced in 4?℃ water led to higher HSP70mRNA expression. Conclusion: Repeated stress may induce the habituation of the HSP70mRNA expression, and intense stress may reverse the habituation induced by repeated chronic stress.

Key words: Coercion, Swimming, Stress, Hippocampus, HSP70mRNA, Habituation, Rats, Wistar

邵淑红,李尊岭,江虹,吴江,潘芳 . 不同强度和时程应激对大鼠海马HSP70mRNA习惯化表达的影响[J]. 山东大学学报(医学版), 2006, 44(8): 770-773.

SHAO Shu-hong,LI Zun-ling,JIANG Hong,WU Jiang,PAN Fang. Stress in different intensity and duration effects the habitual expression of HSP70mRNA on the hippocampus of rats[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2006, 44(8): 770-773.

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