CTLA-4Ig抑制再生障碍性贫血患者T淋巴细胞

山东大学学报(医学版)

CTLA-4Ig抑制再生障碍性贫血患者T淋巴细胞

隋潇徽，徐从高，李丽珍，张锑，于媛，彭军

山东大学齐鲁医院血液科，山东济南 250012

收稿日期:2005-09-09 修回日期:1900-01-01 出版日期:2006-05-24 发布日期:2006-05-24
通讯作者: 隋潇徽

SUIXiao-hui,XUYi-gao,LILi-zhen,ZHANGDi,YUYuan.PENGJun

Received:2005-09-09 Revised:1900-01-01 Online:2006-05-24 Published:2006-05-24
Contact: SUIXiao-hui

摘要/Abstract

摘要： 研究细胞毒性T淋巴细胞相关抗原4融合蛋白（CTLA4Ig）诱导再生障碍性贫血（AA）患者骨髓T淋巴细胞免疫耐受的作用。方法：采用免疫荧光标记法、流式细胞术分析CTLA4Ig阻断前后AA患者骨髓T淋巴细胞表型CD3+、CD4+、CD8+、CD25+、CD4+CD25+和CD8+CD25+的变化。结果：再障试验组与对照组相比，重型再障（SAA）和非重型再障(NSAA)患者骨髓CD3+、CD25+比值、CD4+和CD8+细胞中CD25+细胞比值均明显减低(P＜0.05),CTLA4Ig对重型再障的抑制作用高于非重型再障，对CD4+细胞的抑制作用高于CD8+群体。结论：CTLA4Ig能够抑制再生障碍性贫血患者骨髓局部的异常自身免疫反应，降低骨髓T淋巴细胞对丝裂原的异常反应。

Abstract: o investigate the inhibition of the PHAinduced abnormal bone marrow T lymphocyte proliferation and activation by the CTLA4Ig fusion protein in vitro in patients with aplastic anemia (AA). Method: Flow cytometry and immunofluorescence were used to analyze the mononuclear cells from bone marrow of the aplastic anemia patients. Result: In contrast to the controls, the expressions of the CD3, CD25, CD25 on CD4+, CD8+ lymphocytes were statistically decreased(P＜0.05) in both serious and nonserious AA. Conclusion: The CTLA4Ig fusion protein can induce the T cell tolerance and inhibit the T cell abnormal proliferation and activation in patients with AA.

Key words: Anemia, aplastic, Tlymphocytes, cytotoxic, Immune tolerance

隋潇徽,徐从高,李丽珍,张锑,于媛,彭军. CTLA-4Ig抑制再生障碍性贫血患者T淋巴细胞[J]. 山东大学学报(医学版), 2006, 44(5): 485-488.

SUIXiao-hui,XUYi-gao,LILi-zhen,ZHANGDi,YUYuan.PENGJun. [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2006, 44(5): 485-488.

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