Abstract: Objective:To study the role of tumorstromal fibroblasts(TSF) on the transcription and expression of human leukocyte antigen A (HLAA) and CD86. Methods: Tca8113 cells (oral tongue squamous carcinoma cell line) were cocultivated with TSF in a noncontact manner by Millicell chamber, and then the transcription and the expression of HLAA, HLA B, HLAG and CD86 of Tca8113 cells were detected by semiquantitive RTPCR, Western blotting and flow cytometry analysis. Results: The transcripts of HLAA and CD86 of Tca8113 were downregulated by TSF to 66.49% and 52.31%, respectively compared with the control (P<0.05); But HLAB and HLAG were not influenced almost. Conclusion: TSF downregulates the expression of HLAA and CD86 in oral squamous cells, and therefore the escape from immune may result in tumor progression and metastasis in vivo.

Key words: Mouth neoplasms, HLA antigens, Antigens, CD86, Stromal fibroblasts