人参皂苷对金黄色葡萄球菌生物被膜形成的影响

doi:10.6040/j.issn.1671-7554.0.2018.013

摘要/Abstract

摘要： 目的探讨人参皂苷Rh2单体对金黄色葡萄球菌(S.aureus)生物被膜形成的影响。方法通过96孔细胞培养板构建S.aureus生物被膜,探讨Rh2对生物被膜形成的影响;通过RNA提取及实时荧光定量PCR检测,探讨Rh2对S.aureus黏附素相关基因表达量的影响;将Rh2与抗生素环丙沙星(CIP)、庆大霉素(GEN)和万古霉素(VAN)联用,采用96孔细胞培养板结合结晶紫染色法探讨其与抗生素之间是否存在协同抑制生物被膜形成的能力。结果 Rh2对S.aureus浮游菌的增殖无影响,但5 μg/mL的Rh2能显著降低S.aureus生物被膜的形成量(P<0.05),且具有剂量依赖性;10 μg/mL的Rh2能显著抑制S.aureus黏附相关基因icaA、icaC和icaD的表达(P均<0.05);此外,10 μg/mL的Rh2可显著促进亚抑菌浓度CIP、GEN和VAN抑制S.aureus生物被膜的形成能力(P均<0.01)。结论人参皂苷Rh2单体能通过抑制S.aureus黏附相关基因icaA、icaC和icaD的表达而抑制其生物被膜的形成,并能显著促进CIP、GEN和VAN抑制S.aureus生物被膜形成的能力。

关键词: 金黄色葡萄球菌, 人参皂苷, Rh2, 生物被膜, 协同作用

Abstract: Objective To investigate the effect of ginsenoside Rh2 on the biofim formation of Staphylococcus aureus(S.aureus). Methods The biofilm was constructed by using 96-well cell culture plate to study the effect of Rh2 on the biofilm formation of S.aureus, and the effect of Rh2 on the expression of adhesin-related genes of S.aureus was evaluated by RNA extraction and real-time quantitative PCR. The combination of 96-well cell culture plate with crystal violet staining was used to explore the synergistic antibiofilm effects of Rh2 in combination with ciprofloxacin(CIP), gentamicin(GEN)and vancomycin(VAN). Results Rh2 had no effect on the cell growth of S.aureus, while could significantly reduce the biofilm formation(P<0.05)at the concentration of 5 μg/mL. Rh2(10 μg/mL)could significantly inhibit the gene expressions of icaA, icaC and icaD(P<0.05), and promote the inhibitory effects of CIP, GEN and VAN on S.aureus biofilm formation(P<0.05). Conclusion Ginsenoside Rh2 can inhibit the biofilm formation of S. aureus by inhibiting the expressions of adhesion-related genes icaA, icaC and icaD. Rh2 can also promote the inhibitory effects of CIP, GEN and VAN on the biofilm formation of S.aureus.

Key words: Staphylococcus aureus, Ginsenosides, Rh2, Biofilm, Synergistic effect

中图分类号:

R378.1+1

安继红,吕维玲,张永州,韩姗姗,刘强. 人参皂苷对金黄色葡萄球菌生物被膜形成的影响[J]. 山东大学学报 (医学版), 2018, 56(7): 28-32.

AN Jihong, L(¨overU)Weiling, ZHANG Yongzhou, HAN Shanshan, LIU Qiang. Effects of ginsenosides on the biofilm formation of Staphylococcus aureus [J]. Journal of Shandong University (Health Sciences), 2018, 56(7): 28-32.

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