JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)

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Radiosensitivity enhancement by NS-398, a cyclooxygenase(COX)-2 selective inhibitor, via enhancing the sensitivity to X-ray irradition-induced apoptosis on the hunman colorectal cancer cell line HT-29

WANG Zhen-bo1,3，HU Li-kuan1,2，ZHU Xin-li1，SONG Yi-peng4   

  1. 1.Cancer Center, Qilu Hospital of Shandong University；2.School of Medicine, Shandong University；2.School of Medicine, Shandong University；4.Department of Oncology, Yuhuangding Hospital
  • Received:2006-05-26 Revised:1900-01-01 Online:2007-02-24 Published:2007-02-24
  • Contact: HU Li-kuan

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Abstract: Objective： To investigate the radiosensitizing effect and mechanisms of COX-2 inhibitor NS-398 on the colorectal cancer cell line HT-29. Methods: The colorectal cancer cell line HT-29 had been incubated with 25μmol/L NS-398 for 24h before X-ray irradiation at different doses(0, 2, 4, 6 and 8Gy). The cells were assayed for clonogenic survival to determine the radiosensitizing effect of NS-398. HT-29 cell apoptosis was confirmed by DNA fragmentation (DNA ladder, sub-G1 formation). Caspase 3, 8 and 9 activation were measured by spectrophotometry. Results： The sensitization enhancement ratios in HT-29 cells were 1.36 and 1.27 according to Dq and D0 respectively. The typical DNA “Ladder" and higher sub-G1 cell peak were observed in the NS-398 pre-treatment group after irradiation. Caspase3, 8 and 9 activation of the HT-29 cells with pretreatment of 25μmol/L NS-398 for 24h increased after irradiation, especially caspase 3 and 9. Conclusion： The COX-2 inhibitor NS-398 has a radiosensitizing effect on HT-29 cells， and this mechanism may be closely associated with the increase of the sensitivity of HT-29 to X-ray irradiationinduced apoptosis.

Key words: Cyclooxygenase inhibitors, Radiosensitivity, Apoptosis, Caspase

CLC Number: 

  • R735.3
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