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The VMAT2transgenic CHO cells resist dopamine toxicity

WANG Ming-wei1,2，GU Ping1,2，LIU Li2，WANG Yan-yong1,2，CUI Dong-sheng2   

  1. 1.Department of Neurology, First Hospital of Hebei Medical University, Shijiazhuang 050031；2.Hebei Laboratory of Brain Aging and Cognitive Neuroscience, Shijiazhuang050031
  • Received:2005-08-31 Revised:1900-01-01 Online:2007-02-24 Published:2007-02-24

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Abstract: Objective: To study the resistance of VMAT2 transgenic CHO cells to the toxicity of dopamine. Methods: Immunofluorescence was used to detect the expression of VMAT2; MTT assay was used to detect the viability of wild type china hamster ovary cells (WT-CHOs) and the VMAT2 transgeneic CHO cells (VMAT2-CHOs) with different concentrations of dopamine; meanwhile ELISA assay and ROS kit were respectively used to measure the dopamine level and reactive oxygen species of the cytoplasm. Result: The result indicated that there was an apparent green fluorescence at the circumference of the nucleus in VMAT2-CHO, but there was no fluorescence in WT-CHO. Being acted upon by dopamine for 72h with the scope of 0.25-0.4mmol/L, the viability of VMAT2-CHO was higher than that of WT-CHO (P<0.05); the dopamine level of VMAT2-CHO(199.33±15.155?ng/ml) was higher than that of WT-CHO(141.4±8.784ng/ml)(P<0.01) at 24 hours after being affected by dopamine, while the ROS decreased from 217.895±15.885 U/106cells in WT-CHO to 144.490±5.295 U/106 cells in VMAT2-CHO (P<0.01). Conclusion: VMAT2 transgenic CHO cells can resist the toxicity of dopamine.

Key words: Vesicular monamine transporter-2, Dopamine, Reactive oxygen species, Chinese hamster ovary cell

CLC Number: 

  • R742.5
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