Journal of Shandong University (Health Sciences) ›› 2017, Vol. 55 ›› Issue (12): 13-17.doi: 10.6040/j.issn.1671-7554.0.2017.163

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Effects of Vitamin D on the content of Ceramide in rat adrenal pheochromocytoma PC12 Cells

GUAN Xinyuan, GAO Jifang, GUO Juanjuan, LIU Shilian   

  1. Department of Biochemistry and Molecular Biology, School of Basic Medicine, Shandong University, Jinan 250012, Shandong, China
  • Online:2017-12-20 Published:2022-09-27

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Abstract: Objective To invastigate the effect of vitamin D(VD)on the content of ceramide(Cer)in rat adrenal pheochromocytoma PC12 cells. Methods MTT was performed to verify the effect of short-chain C2-Cer(20 μmol/L)on cell proliferation. High performance liquid chromatography tandem mass spectrometry(HPLC-MS)was performed to detect the concentration of C16: 0-Cer in PC12 cells stimulated with VD(50 or 0 nmol/L). qRT-PCR was performed to measure the mRNA expression levels of enzymes which were related to the metabolism of Cer in PC12 cells after being stimulated with VD(50 or 0 nmol/L). Western blotting was performed to measure the protein levels of SPTLC1 and Cers6 in PC12 cells that were stimulated with VD(200, 100, 50, 25, 12.5 and 0 nmol/L). Results Activity of PC12 cells was inhibited by C2-Cer with IC50 being 2.800 μmol/L. Compared with VD at 0 nmol/L, the content of C16: 0-Cer in PC12 cells was decreased by VD at 50 nmol/L ［(19.37±0.61)ng/mL vs(16.50±0.10)ng/mL, P=0.010］, the expressions of SPTLC1 mRNA(P=0.001)and Cers6 mRNA(P<0.001)in PC12 cells were increased, the protein expression of SPTLC1 in PC12 cells was reduced(P=0.032)and the protein expression of Cers6 was increased(P=0.020). Conclusion The concentration of C16:0-Cer is decreased in PC12 cells that were treated with VD, and it may be related to the decrease of protein expression of SPTLC1.

Key words: Ceramide, PC12 cell, Vitamin D, High pressure liquid chrometography /mass spectroscopy

CLC Number: 

  • R744.5+1
[1] Grigoriadis N, Van Pesch V. A basic overview of multiple sclerosis immunopathology[J]. Eur J Neurol, 2015, 22(2):3-13.
[2] Dyment DA, Yee IM, Ebers GC. Multiple sclerosis in stepsiblings: recurrence risk and ascertainmen[J]. J Neurol Neurosurg Psychiatry, 2006, 77(2):258-259.
[3] Beecham AH, Patsopoulos NA, Xifara DK. International multiple slerosis genetics consortium(IMSGC), analysis of immune-related loci identifies 48 new susceptibility variants for multiple sclerosis[J]. Nat Genet, 2013, 45(11):1353-1360.
[4] Cross AH, Naismith RT. Established and novel disease-modifying treatments in multiple sclerosis[J]. J Intern Med, 2014, 275(4):350-363.
[5] Smolders J, Damoiseaux J, Menheere P. Vitamin D as an immune modulator in multiple sclerosis, a review[J]. J Neuroimmunology, 2008, 194(1): 7-17.
[6] Scott TF, Hackett CT, Dworek DC. Low vitamin D level is associated with higher relapse rate in natalizumab treated MS patients[J]. J Neurol Sci, 2013, 330(1-2): 27-31.
[7] Martinelli V, Dalla Costa G, Colombo B. Vitamin D levels and risk of multiple sclerosis in patients with clinically isolated syndromes[J]. Mult Scler, 2014, 20(2):147-155.
[8] Spach KM, Hayes CE. Vitamin D3 confers protcttion from autimmune encephalomyelit is only in female mice[J]. J Immunol, 2005, 175(6):4119-4126.
[9] Gomez-Mu(~overn)oz A, Presa N, Gomez-Larrauri A. Control of inflammatory responses by ceramide, sphingosine 1-phosphate and ceramide 1-phosphate[J]. Prog Lipid Res, 2016, 61:51-62. doi: 10.1016/j.plipres.2015.09.002.
[10] Dai L, Trillo-Tinoco J, Bai A, et al. Ceramides promote apoptosis for virus-infected lymphoma cells through induction of ceramide synthases and viral lytic gene expression[J]. Oncotarget, 2015, 6(27): 24246-24260.
[11] Saddoughi SA, Ogretmen B. Diverse functions of ceramide in cancer cell death and proliferation[J]. Adv Cancer Res, 2013, 117:37-58. doi: 10.1016/B978-0-12-394274- 6.00002-9.
[12] Susanne S, Ferreiros N, Birod K. Ceramide synthase 6 plays a critical role in the development of experimental autoimmune encephalomyelitis[J]. J Immunol, 2012, 188(11):5723-5733.
[13] Zhu Y, Qin Z, Gao J. Vitamin D therapy in experimental allergic encephalomyelitis could be limited by opposing effects of sphingosine-1-phosphate and gelsolin dysregulation[J]. Mol Neurobiol, 2014, 50(3):733-743.
[14] Hoveizi E, Tavakol S, Ebrahimi-Barough S. Neuroprotective effect of transplanted neural precursors embedded on PLA/CS scaffold in an animal model of multiple sclerosis[J]. Mol Neurobiol, 2015, 51(3):1334-1342.
[15] Ohno Yusuke, Nakamichi S, Ohkuni A. Essential role of the cytochrome P450 CYP4F22 in the production of acylceramide, the key lipid for skin permeability barrier formation[J]. PNAS, 2015, 112(25):7707-7712.
[16] Macchi B, Marino-Merlo F, Nocentini U, et al. Role of inflammation and apoptosis in multiple sclerosis: comparative analysis between the periphery and the central nervous system[J]. J Neuroimmunol, 2015, 287:80-87. doi:10.1016/j.jneuroim.2015.08.016.
[17] Hartfield PJ, Manyne GC, Murray AW. Ceramide induces apoptosis in PC12 cells[J]. FEBS Letters, 1997, 401(2-3):148-152.
[18] Ben-David O, Futerman AH. The role of the ceramide acyl chain length in neurodegeneration: involvement of ceramide synthases[J]. Neuromolecular Med, 2010, 12(4):341-350.
[19] Hojjati MR, Li Z, Jiang XC. Serine palmitoyl-CoA transferase(SPT)deficiency and sphingolipid levels in mice[J]. Biochim Biophys Acta, 2005, 1737(1):44-51.
[20] Michael M, Sarah S. Sphingolipid metabolites in inflammatory disease[J]. Nature, 2014, 510(7503): 58-67.
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