Journal of Shandong University (Health Sciences) ›› 2017, Vol. 55 ›› Issue (12): 18-23.doi: 10.6040/j.issn.1671-7554.0.2017.658

Previous Articles     Next Articles

Effect of myosin light chain kinase on airway inflammation and lung function in asthma model mice

GONG Xiaodan1,2, ZHAO Fangzheng2,3, CAO Ke1,2, DENG Penghui2, ZHANG Caiqing2   

  1. 1. Medical College of Shandong University, Jinan 250012, Shandong, China;
    2. Respiratory Physicians of Qianfoshan Hospital Affiliated to Shandong University, Jinan 250014, Shandong, China;
    3. The Second Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250001, Shandong, China
  • Online:2017-12-20 Published:2022-09-27

PDF (PC)

341

Abstract: Objective To study the effect of myosin light chain kinase(MLCK)on airway inflammation and pulmonary function through the determination of its expression in asthmatic mice airway. Methods Twenty female BALB/c mice were randomly divided into normal control group(n=10)and asthma group(n=10). Mice model of asthma was established by using chicken ovalbumin(OVA). The counts of leukocytes and eosinophilic granulocytes were observed with optical microscope. The expressions of the inflammatory mediators as IL-4 and IFN-γ in bronchoalveolar lavage fluid(BALF)were detected by ELISA kit. The expression of MLCK in the lung tissue of asthmatic mice was detected by Western blotting. The expression of MLCK in the gene level was detected by qPCR. Results The lung function test showed that peak expiratory flow(PEF), the ratio of forced expiratory volume in 0.4 second to forced vital capacity(FEV0.4/FVC)in asthma group was lower than those in normal control group(P<0.001). The counts of leukocytes and eosinophilic granulocytes in asthma group were higher than those in normal control group(P<0.001). The IL-4 level in the BALF in asthma group was higher than that in normal control group(P<0.001), while the IFN-γ level 山 东 大 学 学 报 (医 学 版)55卷12期 -宫晓丹,等.肌球蛋白轻链激酶对哮喘小鼠气道炎症及肺功能的影响 \=-was lower than that in nornal control group(P<0.001). The expression of MLCK in the lung tissue of asthma group was significantly increased(P=0.006). The expression of MLCK in the gene level of asthma group was higher compared with the normal control group(P<0.001). Conclusion MLCK may be involved in the airway inflammation response and influence the lung function.

Key words: Asthma, Myosin light chain kinase, Lung function, IL-4, IFN-γ

CLC Number: 

  • R562.2+5
[1] Wang Y, Bai C, Li K, et al. Role of airway epithelial cells in development of asthma and allergic rhinitis[J]. Respir Med, 2008, 102(7): 949-955.
[2] Deckers J, Branco Madeira F, Hammad H. Innate immune cells in asthma[J]. Trends Immunol, 2013, 34(11): 540-547.
[3] Luzina IG, Lockatell V, Lavania S, et al. Natural production and functional effects of alternatively spliced interleukin-4 protein in asthma[J]. Cytokine, 2012, 58(1): 20-26.
[4] Liu Y, Zhuo A, Liu W, et al. The-33C/T polymorphism in the interleukin 4 gene is associated with asthma risk: a meta-analysis[J]. J Investig Allergol Clin Immunol, 2014, 24(2): 114-121.
[5] 于华凤,王广新,韩玉玲,等. 儿童支气管哮喘血清IL-4、IL-10和IFN-γ浓度的测定及其临床意义[J]. 中华哮喘杂志(电子版), 2010, 4(5): 320-323. YU Huafeng, WANG Guangxin, HAN Yuling, et al. Levels of serum IL-4, IL-10, IFN-γ in children with bronchial asthma and their clinical significance[J]. Chin J Asthma(Electronic Edition), 2010, 4(5): 320-323.
[6] Ma X, Cheng Z, Kong H, et al. Changes in biophysical and biochemical properties of single bronchial smooth muscle cells from asthmatic subjecrs[J]. Am J of Physiol Lung Cell Mol Physiol, 2002, 283(6):1181-1189.
[7] Bousquet J, Jeffery PK, Busse WW, et al. Asthma. From bronchoconstriction to airways inflammation and remodeling[J]. Am J Respir Crit Care Med, 2000, 161(5): 1720-1745.
[8] Lambert RK, Wiggs BR, Kuwano K, et al. Functional significance of increased airway smooth muscle in asthma and COPD[J]. J Appl Physiol, 1993, 74(6): 2771-2781.
[9] Chen C, Kudo M, Rutaganira F, et al. Integrin α9β1 in airway smooth muscle suppresses exaggerated airway narrowing[J]. J Clin Invest, 2012, 122(8): 2916-2927.
[10] Kudo M, Melton AC, Chen C, et al. IL-17A produced by αβ T cells drives airway hyper-responsiveness in mice and enhances mouse and human airway smooth muscle contraction[J]. Nat Med, 2012, 18(4): 547-554.
[11] 赵彩霞. 支气管哮喘发病机制的研究进展[J]. 医学理论与实践, 2016, 29(7): 870-872.
[12] 李文娟,高超. 支气管哮喘的变态反应机制[J]. 临床医药实践, 2013, 22(1): 44-46.
[13] Romagnani S. Immunologic influences on allergy and the TH1/TH2 balance[J]. J Allergy and Clin Immunol, 2004, 113(3): 395-400.
[14] 曲书强,张磊,吴静,等.干扰素-γ对小鼠哮喘模型治疗的研究[J]. 现代生物医学进展, 2009, 9(2): 230-231. QU Shuqiang, ZHANG Lei, WU Jing, et al. A study on IFN-γ in treatment of asthma mice[J]. Progress in Modern Biomedicine, 2009, 9(2): 230-231.
[15] Packard KA. Effects of histamine on Th1/Th2 cytokine balance[J]. Int Immunopharmacol, 2003, 3(7): 909-920.
[16] Wu C, Yang G, Bermúdez-Humarán LG, et al. Immunomodulatory effects of IL-12 secreted by Lactococcus lactis on Th1/Th2 balance in ovalbumin(OVA)-induced asthma model mice[J]. Int Immunopharmacol, 2006, 6(4): 610-615.
[17] Shen H, Xia L, Lu J, et al. Interleukin-4 in rheumatoid arthritis patients with interstitial lung disease: a pilot study[J]. Indian J Med Res, 2013, 138(6): 919-921.
[18] Luzina IG, Lockatell V, Todd NW, et al. Alternatively spliced variants of interleukin-4 promote inflammation differentially[J]. J Leukoc Biol, 2011, 89(5): 763-770.
[19] Zhang HF, Li TB, Liu B, et al. Inhibition of myosin light chain kinase reduces NADPH oxidase-mediated oxidative injury in rat brain following cerebral ischemia/reperfusion[J]. Naunyn Schmiedebergs Arch Pharmacol, 2015, 388(9): 953-963.
[20] 易在凤,范恒,杨佳.肌球蛋白轻链激酶在炎症性肠病中的作用[J]. 世界华人消化杂志, 2014, 22(35): 5467-5472. YI Zaifeng, FAN Heng, YANG Jia. Role of myosin light chain kinase in inflammatory bowel disease[J]. World Chinese Journal of Digestology, 2014, 22(35): 5467-5472.
[21] Schmidt D, Rabe KF. Immune mechanisms of smooth muscle hyperreactivity in asthma[J]. J Allergy Clin Immunol, 2000, 105(4): 673-682.
[22] 孙艳宏. 气道平滑肌细胞中GLP-1受体的表达对细胞增殖及炎症因子的调节机制研究[D]. 广州:南方医科大学, 2016.
[23] 张贞贞,莫碧文.气道平滑肌细胞作为免疫调节细胞与支气管哮喘炎症的关系[J]. 中华哮喘杂志(电子版), 2012, 6(1): 39-43. ZHANG Zhenzhen, MO Biwen. Airway smooth muscle cell as an immunomodulatory cell in inflammatory reaction in bronchial asthma[J]. Chin J Asthma(Electronic Edition), 2012, 6(1): 39-43.
[24] Howarth PH, Knox AJ, Amrani Y, et al. Synthetic responses in airway smooth muscle.[J]. J Allergy Clin Immunol, 2004, 114(114): 32-50.
[25] Damera G, Tliba O, Panettieri RA, et al. Airway smooth muscle as an immunomodulatory cell[J]. Pulm Pharmacol Ther, 2009, 22(5): 353-359.
[1] ZHANG Qiuping, ZHU Huizhi, LYU Chuan, XIA Yongqi, ZHANG Xiu. Identification of potential key autophagy- and ferroptosis-related genes in asthma based on bioinformatics analysis [J]. Journal of Shandong University (Health Sciences), 2026, 64(1): 74-87.
[2] ZHANG Zheng, WANG Jianwei, YANG Yujuan, ZHANG Yu, SONG Xicheng. Immunoglobulin E changes and risk factors in asthma children between 2008 and 2019 [J]. Journal of Shandong University (Health Sciences), 2025, 63(7): 32-36.
[3] CAO Luofei, WANG Shanshan, WANG Jinrong, JIANG Heyun, MIAO Yu, MA Guangzeng. Analysis of the characteristics of bronchial dilation test in children with FeNO elevation during asthma exacerbation [J]. Journal of Shandong University (Health Sciences), 2025, 63(6): 38-44.
[4] SUN Congcong, CUI Wenjing, ZHANG Jintao, ZHANG Dong, LIU Xiaofei, PAN Yun, QI Qian, XU Jiawei, ZENG Rong, GUO Hongxi, DONG Liang. Roles of ferroptosis in asthmatic airway remodeling [J]. Journal of Shandong University (Health Sciences), 2024, 62(7): 1-9.
[5] WANG Jing, LIU Xiaofei, ZENG Rong, XU Changjuan, ZHANG Jintao, DONG Liang. Identification of necroptosis-related biomarkers in asthma based on machine learning algorithms [J]. Journal of Shandong University (Health Sciences), 2024, 62(7): 21-32.
[6] YAN Jinyan, YANG Chun, LI Lei, WU Fuling, JIAO Yongli, ZHANG Xiaowei, LI Jing, ZHANG Ruizhen, WANG Lei, MA Xiang. Correlation between asthma and pertussis infection in children of Shandong Province, China [J]. Journal of Shandong University (Health Sciences), 2024, 62(7): 33-41.
[7] ZHANG Jintao, DONG Liang. Airway epithelium and epithelial-derived cytokines in asthma: reflection and outlook [J]. Journal of Shandong University (Health Sciences), 2024, 62(5): 1-6.
[8] DING Yiren, LIU Wanying, YAO Lei, YAO Xin. Research progress of the treatment of asthma with macrolide antibiotics [J]. Journal of Shandong University (Health Sciences), 2024, 62(5): 21-27.
[9] WANG Ting, ZHANG Li, WANG Gang. Neuropsychological asthma [J]. Journal of Shandong University (Health Sciences), 2024, 62(5): 28-34.
[10] XU Fang, TIAN Guoxiong, SUN Beibei, CHEN Xinyi, CHEN Gaoying, ZHANG Ruiqi, YING Songmin, WU Miaolian, ZHANG Chao, WU Youqian. Research progress on biological and cellular therapies for severe asthma [J]. Journal of Shandong University (Health Sciences), 2024, 62(5): 35-42.
[11] SHI Shuochuan, ZENG Rong, ZHANG Jintao, ZHANG Dong, PAN Yun, LIU Xiaofei, XU Changjuan, WANG Ying, DONG Liang. Bioinformatics-based exploration of potential differential immune genes and immune infiltration signatures in bronchial asthma [J]. Journal of Shandong University (Health Sciences), 2024, 62(5): 43-53.
[12] XU Xinjun, SHAO Liting, CHEN Ying, LIU Huifang, YANG Yujuan, ZHANG Yu, WANG Hanrui, SONG Xicheng. Comparison of the therapeutic effect of SYN008 with Xolair® in allergic asthma mice: inflammation and remodeling [J]. Journal of Shandong University (Health Sciences), 2024, 62(12): 1-10.
[13] HAO Yan, CUI Limei, CHEN Ying, YANG Yujuan, SONG Xicheng. Advances of metabolomics in airway inflammatory diseases [J]. Journal of Shandong University (Health Sciences), 2024, 62(12): 117-124.
[14] BU Meiling, WANG Jinrong, FENG Mei, SUN Lifeng. Mechanism of FOXM1 in acute exacerbation of asthma induced by respiratory virus infection in mice [J]. Journal of Shandong University (Health Sciences), 2023, 61(6): 1-9.
[15] ZHANG Qian, QIN Mingming, HE Xuejia, CAI Qiujing, ZHANG Yamin, LI Qingsu, ZHU Weiwei. Effects of calcitriol on EMT induced by TGF-β1 in asthma [J]. Journal of Shandong University (Health Sciences), 2021, 59(7): 10-18.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd