Journal of Shandong University (Health Sciences) ›› 2017, Vol. 55 ›› Issue (12): 7-12.doi: 10.6040/j.issn.1671-7554.0.2017.150

Previous Articles     Next Articles

Expression of Cyr61 in the adipose tissue of obese mice induced by high fat diet

QING Li, HE Tianyi, QIAO Jingting, CUI Chen, HOU Xinguo, CHEN Li   

  1. Department of Endocrinology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Online:2017-12-20 Published:2022-09-27

PDF (PC)

143

Abstract: Objective To investigate the expression and distribution of cysteine rich protein 61(Cyr61)in the adipose tissue of obese mice and its relationship with fat chronic inflammation. Methods A total of 30 male C57BL/6 mice were randomly divided into the normal diet group(NC group)and high fat diet group(HFD group). The HFD group was fed with high fat diet of 60% fat content to construct the obese mouse models. The NC group was fed with normal diet. After modeling, the body fat content of mice was measured, glucose tolerance and blood lipids were detected with intraperitoneal injection of glucose tolerance test(IPGTT), the expression and distribution of Cyr61 in the testis tissue were measured, and the expressions of tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6)mRNA in the adipose tissue were detected. Results The body weight, body fat content and fat tolerance of obese mice were significantly higher than those in NC group(P<0.05). The serum cholesterol and low density lipoprotein cholesterol were significantly higher than those in NC group(P<0.05). The expressions of TNF-α and IL-6 in the adipose tissue of obese mice were up-regulated(P<0.05). Both of the protein and mRNA levels of Cyr61 increased in the adipose tissue of HFD group(P<0.05), and Cyr61 was mainly distributed in the adipose tissue matrix. Conclusion Cyr61 expression, which is mainly located in the adipose tissue matrix, is significantly increased in the adipose tissue of obese mice, and is consistent with the expression of inflammatory factors, suggesting that it may be related to the occurrence of chronic inflammation.

Key words: Cysteine rich protein, Adipose tissue, Obesity, Inflammatory factor, Chronic inflammation

CLC Number: 

  • R723.14
[1] Bi Y, Xu Y, Ning G. Prevalence of diabetes in Chinese adults-reply[J]. JAMA, 2014, 311(2): 200-201.
[2] Hotamisligil GS. Inflammation and metabolic disorders[J]. Nature, 2006, 444(7121): 860-867.
[3] Chawla A, Nguyen KD, Goh YP. Macrophage-mediated inflammation in metabolic disease[J]. Nat Rev Immunol, 2011, 11(11): 738-749.
[4] Lumeng CN, Saltiel AR. Inflammatory links between obesity and metabolic disease[J]. J Clin Invest, 2011, 121(6): 2111-2117.
[5] Shu CJ, Benoist C, Mathis D. The immune systems involvement in obesity-driven type 2 diabetes[J]. Semin Immunol, 2012, 24(6): 436-442.
[6] Obrien TP, Yang GP, Sanders L, et al. Expression of cyr61, a growth factor-inducible immediate-early gene[J]. Mol Cell Biol, 1990, 10(7): 3569-3577.
[7] Lin J, Huo R, Xiao L, et al. A novel p53/microRNA-22/Cyr61 axis in synovial cells regulates inflammation in rheumatoid arthritis[J]. Arthritis Rheumatol, 2014, 66(1): 49-59.
[8] Hotamisligil GS, Shargill NS, Spiegelman BM. Adipose expression of tumor necrosis factor-alpha: direct role in obesity-linked insulin resistance[J]. Science, 1993, 259(5091): 87-91.
[9] Li P, Lu M, Nguyen MT, et al. Functional heterogeneity of CD11c-positive adipose tissue macrophages in diet-induced obese mice[J]. J Biol Chem, 2010, 285(20): 15333-15345.
[10] Kintscher U, Hartge M, Hess K, et al. T-lymphocyte infiltration in visceral adipose tissue: a primary event in adipose tissue inflammation and the development of obesity-mediated insulin resistance[J]. Arterioscler Thromb Vasc Biol, 2008, 28(7): 1304-1310.
[11] Sell H, Habich C, Eckel J. Adaptive immunity in obesity and insulin resistance[J]. Nat Rev Endocrinol, 2012, 8(12): 709-716.
[12] Lumeng CN, Maillard I, Saltiel AR. T-ing up inflammation in fat[J]. Nat Med, 2009, 15(8): 846-847.
[13] Winer S, Chan Y, Paltser G, et al. Normalization of obesity-associated insulin resistance through immunotherapy[J]. Nat Med, 2009, 15(8): 921-929.
[14] Lau LF, Nathans D. Identification of a set of genes expressed during the G0/G1 transition of cultured mouse cells[J]. EMBO J, 1985, 4(12): 3145-3151.
[15] Obrien TP, Lau LF. Expression of the growth factor-inducible immediate early gene cyr61 correlates with chondrogenesis during mouse embryonic development[J]. Cell Growth Differ, 1992, 3(9): 645-654.
[16] Jay P, Berge-Lefranc JL, Marsollier C, et al. The human growth factor-inducible immediate early gene, CYR61, maps to chromosome 1p[J]. Oncogene, 1997, 14(14): 1753-1757.
[17] Leng E, Malcolm T, Tai G, et al. Organization and expression of the Cyr61 gene in normal human fibroblasts[J]. J Biomed Sci, 2002, 9(1): 59-67.
[18] 王志敏, 丛雅琴, 马立宁, 等. Cyr61基因与慢性粒细胞白血病的相关性研究[J]. 山东大学学报(医学版), 2009, 47(10): 94-97. WANG Zhimin, CONG Yaqin, MA Lining, et al. Correlation between Cyr61 and chronic myelogenousleukemis[J]. Journal of Shandong University(Health Sciences), 2009, 47(10): 94-97.
[19] 牛志宏, 高德轩, 丁森泰, 等. Cyr61、CTGF、NOV、WISP-1基因在肾癌中的表达[J]. 山东大学学报(医学版), 2008, 46(7): 701-703. NIU Zhihong, GAO Dexuan, DING Sentai, et al. Expression of Cyr61, CTGF, NOV and WISP-1 in renal cell carcinoma[J]. Journal of Shandong University(Health Sciences), 2008, 46(7): 701-703.
[20] Tong X, Okelly J, Xie D, et al. Cyr61 suppresses the growth of non-small-cell lung cancer cells via the beta-catenin-c-myc-p53 pathway[J]. Oncogene, 2004, 23(28): 4847-4855.
[21] Matsumae H, Yoshida Y, Ono K, et al. CCN1 knockdown suppresses neointimal hyperplasia in a rat artery balloon injury model[J]. Arterioscler Thromb Vasc Biol, 2008, 28(6): 1077-1083.
[22] Athanasopoulos AN, Schneider D, Keiper T, et al. Vascular endothelial growth factor(VEGF)-induced up-regulation of CCN1 in osteoblasts mediates proangiogenic activities in endothelial cells and promotes fracture healing[J]. J Biol Chem, 2007, 282(37): 26746-26753.
[23] Zhang Q, Wu J, Cao Q, et al. A critical role of Cyr61 in interleukin-17-dependent proliferation of fibroblast-like synoviocytes in rheumatoid arthritis[J]. Arthritis Rheum, 2009, 60(12): 3602-3612.
[24] Koon HW, Shih DQ, Hing TC, et al. Substance P induces CCN1 expression via histone deacetylase activity in human colonic epithelial cells[J]. Am J Pathol, 2011, 179(5): 2315-2326.
[25] Koon HW, Zhao D, Xu H, et al. Substance P-mediated expression of the pro-angiogenic factor CCN1 modulates the course of colitis[J]. Am J Pathol, 2008, 173(2): 400-410.
[26] Lantz M, Vondrichova T, Parikh H, et al. Overexpression of immediate early genes in active Graves' ophthalmopathy[J]. J Clin Endocrinol Metab, 2005, 90(8): 4784-4791.
[27] Lin J, Zhou Z, Huo R, et al. Cyr61 induces IL-6 production by fibroblast-like synoviocytes promoting Th17 differentiation in rheumatoid arthritis[J]. J Immunol, 2012, 188(11): 5776-5784.
[28] 徐京京, 赵琳, 张晓莉, 等. 类风湿关节炎患者血清CCN1水平变化及意义[J]. 山东医药, 2016, 56(21): 58-59. XU Jingjing, ZHAO Lin, ZHANG Xiaoli, et al. Changes and significance of serum CCN1 in patients with rheumatoid arthritis[J]. Shandong Med J, 2016, 56(21): 58-59.
[29] Zhu X, Xiao L, Huo R, et al. Cyr61 is involved in neutrophil infiltration in joints by inducing IL-8 production by fibroblast-like synoviocytes in rheumatoid arthritis[J]. Arthritis Res Ther, 2013, 15(6): 187.
[30] Schipper HS, Prakken B, Kalkhoven E, et al. Adipose tissue-resident immune cells: key players in immunometabolism[J]. Trends Endocrinol Metab, 2012, 23(8): 407-415.
[31] Shoelson SE, Lee J, Goldfine AB. Inflammation and insulin resistance[J]. J Clin Invest, 2006, 116(7): 1793-1801.
[1] ZHANG Yuan, LI Yang-Min, FENG Rue-Qiu, CHANG Cai-Yun, PAN Hua-Wei, WANG Shu-Mei. Relationship among serum adiponectin level, obesity and insulin resistance [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2209, 47(6): 124-.
[2] ZHAO Wanxia, ZHAN Qunzhang, JIN Ting, LIU Yuxin, QU Chongzheng, WU Jianchun. Mendelian randomization analysis of the causal relationships between gut microbiota, blood metabolites, and obesity [J]. Journal of Shandong University (Health Sciences), 2026, 64(4): 72-82.
[3] XU Ping, ZHANG Xinmei. Mechanisms of pain in endometriosis [J]. Journal of Shandong University (Health Sciences), 2025, 63(10): 1-7.
[4] FENG Yue, YU Yifan, WU Sijia, LI Hongkai, XUE Fuzhong. Mendelian randomization study of visceral adipose tissue and lung diseases [J]. Journal of Shandong University (Health Sciences), 2024, 62(7): 48-55.
[5] WANG Wenqin, GAO Xianlong, GONG Yongfeng, FENG Ke. YBX3 regulates thermogenesis and energy expenditure by regulating UCP1 in brown adipose tissue [J]. Journal of Shandong University (Health Sciences), 2024, 62(6): 38-47.
[6] LIU Wen, FENG Wenjuan, YANG Yang, JI Changli, CHAO Lan. Impact of BMI on cumulative birth rate in patients with normal ovarian response [J]. Journal of Shandong University (Health Sciences), 2024, 62(3): 54-60.
[7] LIANG Zhonghao, ZHUANG Xianghua, HUANG Shan, HAN Xiaolin, HUA Mengyu, JU Liping, CHEN Shihong. Proteomic analysis of brown adipose tissue in diet-induced obese female mice [J]. Journal of Shandong University (Health Sciences), 2024, 62(2): 10-19.
[8] YANG Baozhu, HUANG Shuyuan, YU Xinxin, DENG Yan, HAN Pengxi, LIU Xiaolong, WANG Ximing. Predictive value of perivascular fat attenuation index based on CCTA for revascularization in chronic total occlusion of coronary artery [J]. Journal of Shandong University (Health Sciences), 2024, 62(10): 98-105.
[9] FAN Jiaxu, CHENG Fang, QIAO Junpeng, FEI Haicheng, CHEN Xueyu, ZHAO Yun, JIA Hongying. Mediators or confounders: should obesity be adjusted to estimate the effects of trihalomethanes on thyroid function [J]. Journal of Shandong University (Health Sciences), 2024, 62(1): 102-110.
[10] QI Shaojun, TANG Yanjin, ZHANG Zhengduo, WU Hong, ZHANG Jiacheng, QIN Chuan, LIU Rui, GAO Xibao. Protective effects of supplementing various trace elements on rats with high-sucrose diet [J]. Journal of Shandong University (Health Sciences), 2023, 61(7): 19-26.
[11] ZHANG Runzhi, GU Hui, LI Yani, YANG Shifeng, GAO Yan, WANG Ruopeng, WANG Ximing. Association among pericoronary fat attenuation index, CT high-risk plaque and degree of coronary artery stenosis in 449 patients [J]. Journal of Shandong University (Health Sciences), 2023, 61(1): 27-31.
[12] SUN Jiye, WANG Ziou, SUN Xiaowei, LI Hongtao. Effects of traditional Chinese medicine fumigation combined with extracorporeal shock wave therapy on 72 cases of femoroacetabular impingement syndrome and serum inflammatory factor [J]. Journal of Shandong University (Health Sciences), 2022, 60(4): 76-81.
[13] ZHANG Mingming, GAO Wei, CUI Jia, HUO Lijing, HU Qingchuan, LI Weihao, WANG Chao. Expression and significance of serum miR-33a in patients with stable angina pectoris [J]. Journal of Shandong University (Health Sciences), 2021, 59(6): 64-70.
[14] XING Zhiqun, LI Dejun, ZHAO Bao, XU Chunyang, JI Hongsheng. Correlation analysis of acetylcholinesterase activity and inflammatory factors of 45 elderly patients with postoperative delirium [J]. Journal of Shandong University (Health Sciences), 2021, 59(3): 92-97.
[15] HAN Xiaoting, YU Xia, DONG Laihui, NA Li, NIU Yanling, ZHAO Junli. Effects of evening primrose oil on the metabolism and intestinal flora of obese infertile women [J]. Journal of Shandong University (Health Sciences), 2021, 59(2): 48-54.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd