JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)

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Effect of VLDL on the expression of the human urate transporter

LI Cheng-qian1,2,　ZHAO Jia-jun1,　WANG Xia2,　LI Chang-gui2,　MIAO Zhi-min2   

  1. 1. Department of Endocrinology, Provincial Hospital Affiliated to Shandong University, Jinan 250021, China;2. Department of Endocrinology, Affiliated Hospital of Medical College, Qingdao University
  • Received:2008-01-25 Revised:1900-01-01 Online:2008-06-16 Published:2008-06-16
  • Contact: LI Cheng-qian

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Abstract: To observe the effect of VLDL in different concentrations on the expression of the human urate transporter (hOAT1) gene in HK-2 cells. Methods HK-2 cells were divided into the control (cultured with DMEM), the V1 (cultured with DMEM and 50umol/L VLDL) and the V2 (cultured with DMEM and 400?umol/L VLDL) groups. In each group, 6 bottles of cells were cultured in vitro for 48 hours, and then the relative quantity of hOAT1 mRNA in the HK-2 cells was determined by real-time fluorescent quantitative PCR assay. ResultshOAT1 mRNA could be detected in all samples, and it was significantly higher in the control group than in the other groups, and in the V1 group it was 41.6% of that in the control group and in the V2 group it was only 34.5% of that in the control group. ConclusionsVLDL can downregulate the expression of the hOAT1gene possibly induced by lipodystrophy easily acting with hyperuricemia.

Key words: Lipoproteins, VLDL, Uric acid, Kidney tubules, Cell, cultured, Gene expression

CLC Number: 

  • R589.7
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