JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (8): 44-49.doi: 10.6040/j.issn.1671-7554.0.2015.1185

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Effects of different cryoprotectants and cooling methods on the histology and immunogenicity of heart valves

GONG Yanjie1, WANG Long2, DONG Laidong3, LI Dong1, SONG Guangmin2, JU Xiuli1   

  1. 1. Department of Paediatrics;
    2. Department of Cardiac Surgery;
    3. Body Donation Receiving Station, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Received:2015-11-27 Online:2016-08-10 Published:2016-08-10

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Abstract: Objective To explore the proper cryoprotectants and cooling methods for storing heart valves in liquid nitrogen. Methods Each heart valve from 6 healthy donors were divided into 7 specimens: 1 was observed and tested directly as control group, and other 6 were divided into 3 cryoprotectant groups(Group A, B and C), with 2 specimens in each groups. Two cooling methods were applied, including -80 ℃ direct cooling(G1 groups)and programmed cooling(G2 groups). The histological changes were observed with hematoxylin-eosin staining, Weigert-Van Gieson(ET+VG)staining, and Masson Trichrome staining. The ultrastructural changes were observed with transmission electron microscopy(TEM). The expression of human leukocyte antigen(HLA)was detected with Western blotting and immunohistochemistry. The immunogenicity of each group was compared. Results Cryopreservation injured the organizational structure of valves, while valves stored in DMSO and chondroitin sulfate sodium showed the least lesions and obviously down-regulated expression of HLA-I. Collagen fibrils were less stained, while the orientation was more disorderly in the programmed cooling groups than in the -80 ℃direct cooling groups, but the expression of HLA-I was lower. Conclusion DMSO in combination with chondroitin sulfate sodium can best preserve the heart valves, with the lowest immunogenicity. The method of -80 ℃direct cooling is conducive for the preservation of collagen fibrils, but it produces higher immunogenicity than programmed cooling.

Key words: Transmission electron microscopy, Heart valves, Cryopreservation, Histology, Human leukocyte antigen

CLC Number: 

  • R654.2
[1] Ross DN. Homograft replacement of the aortic valve[J]. Lancet, 1962, 8(2): 487-488.
[2] Dimitrakakis G, von Oppell UO. Aortic valve-sparing root replacement: a continued challenge![J]. Interact Cardiovasc Thorac Surg, 2011, 13(2): 197.
[3] Batlivala SP, Emani S, Mayer JE, et al. Pulmonary valve replacement function in adolescents: a comparison of bioprosthetic valves and homograft conduits[J]. Ann Thorac Surg, 2012, 93(6): 2007-2016.
[4] Ross DN. Application of homografts in clinical surgery[J]. J Card Surg, 1987, 2(1): 175-183.
[5] Du T, Chao L, Zhao S, et al. Successful cryopreservation of whole sheep ovary by using DMSO-free cryoprotectant[J]. J Assist Reprod Genet, 2015, 32(8): 1267-1275.
[6] O'Brien MF, Stafford EG, Gardner MA, et al. A comparison of aortic valve replacement with viable cryopreserved and fresh allograft valves, with a note on chromosomal studies[J]. J Thorac Cardiovasc Surg, 1987, 94(6): 812-823.
[7] Brockbank KG, Song YC. Mechanisms of bioprosthetic heart valve calcification[J]. Transplantation, 2003, 75(8): 1133-1135.
[8] Naik BR, Rao BS, Vagdevi R, et al. Conventional slow freezing, vitrification and open pulled straw(OPS)vitrification of rabbit embryos[J]. Anim Reprod Sci, 2005, 86(3-4): 329-338.
[9] Mandumpal JB, Kreck CA, Mancera RL. A molecular mechanism of solvent cryoprotection in aqueous DMSO solutions[J]. Phys Chem Chem Phys, 2011, 13(9): 3839-3842.
[10] Rich SJ, Armitage WJ. Corneal tolerance of vitrifiable concentrations of propane-1,2-diol[J]. Cryobiology, 1991, 28(2): 159-170.
[11] 周俊, 刘景汉, 欧阳锡林, 等. 血小板胞内海藻糖测定方法的建立和评价[J].中国实验血液学杂志, 2004, 12(6): 305-307
[12] Foreman J, Pegg DE. Cell preservation in a programmed cooling machine: the effect of variations in supercooling[J]. Cryobiology, 1979, 16(4): 315-321.
[13] 胡正强. 可溶性HLA免疫生物学研究进展[J]. 国外医学免疫学分册, 2002, 25(1): 39-41.
[14] Dignan R, O'Brien M, Hogan P, et al. Aortic valve allograft structural deterioration is associated with a subset of antibodies to human leukocyte antigens[J]. J Heart Valve Dis, 2003, 12(3): 382-390.
[15] Simon A, Wilhelmi M, Steinhoff G, et al. Cardiac valve endothelial cells: relevance in the long-term function of biologic valve prostheses[J]. J Thorac Cardiovasc Surg, 1998, 116(4): 609-616.
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