JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (8): 28-33.doi: 10.6040/j.issn.1671-7554.0.2015.1172

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Effect of interleukin-25 on inducing the airway remodeling of asthma mice by nuocyte cells

LIU Qingfa1,2, WANG Chao1,2, SUN Qijing1,2, GONG Xiaodan1,2, ZHANG Caiqing2   

  1. 1. School of Medicine, Shandong University, Jinan 250012, Shandong, China;
    2. Department of Respiratory, Qianfoshan Hospital Affiliated to Shandong University, Jinan 250014, Shandong, China
  • Received:2015-11-26 Online:2016-08-10 Published:2016-08-10

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Abstract: Objective To investigate the effects of interleukin-25(IL-25)on airway remodeling of asthma mice by nuocyte cells. Methods Thirty mice were randomly divided into control mouse group, asthma mouse group, and anti-IL-25 mouse group, ten in each group. Nuocyte cells were collected from the spleens and marrows of asthma mice which were induced by ovalbumin(OVA), and diveded into control cell group, IL-25 cell group, and anti-IL-25 cell group according to the different intervention conditions. The lung tissue pathology was observed by HE staining. The expressions of interleukin-4(IL-4)and interleukin-13(IL-13)proteins in bronchoalveolar lavage fluid(BALF)and nuocyte cells supernatant were detected by ELISA. The numbers of nuocyte cells in BALF were detected by flow cytometry. The expressions of α-smooth muscle actin(α-SMA)and I collagen(collagen-I)were detected with immunohistochemistry, real-time PCR and Western blotting. Results Compared with the control mouse group, expressions of IL-4 and IL-13, numbers of nuocyte cells in BALF, expressions of α-SMA and collagen-I increased in asthma mouse group(P<0.05), while those in anti-IL-25 mouse group had no significant differences(P>0.05). Compared with the control cell group, expressions of IL-4 and IL-13 increased significantly in the cells supernatant in IL-25 cell group(P<0.05), while those in anti-IL-25 group had no significant differences(P>0.05). Conclusion IL-25 may promote the secreting of TH2 cytokines by nuocyte cells, thus leads to the airway remodeling of the asthma mice.

Key words: Airway remodeling, Interleukin-25, Asthma, Nuocyte cell

CLC Number: 

  • R562.25
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