Abstract: Objective To observe the effect of liraglutide on the distribution of leptin and fat in high-fat diet induced C57BL/6 obese mice model. Methods A total of 60 C57BL/6 mice aged 4 weeks, body weight(14.03+0.92)g were randomly divided into 2 groups: high-fat diet(40% fat)group(n=45)and normal chow diet(4% fat)group(n=15). The body weight was recorded once a week until the obese mice models were built. Then the 45 obese mice in the high-fat diet group models were randomly divided into 3 groups: liraglutide group［intraperitoneal injection of 100 μg/(kg·d), n=15 ］, transferred normal diet group［intraperitoneal injection of 0.017 mL/(kg·d), n=15)］ and high fat diet group(n=15). During the 12-week intervention, the body weight of all animals was recorded. The fat content and leptin were monitored by magnetic resonance imaging and enzyme-linked immunosorbent assay. Results The liraglutide group had the lowest body weight than the other 3 groups(P<0.05). The liraglutide group had reduced degree of leptin compared with transferred normal diet group and high fat diet group(P<0.05). The liraglutide group 山 东 大 学 学 报 (医 学 版)54卷8期 -沙莎,等.利拉鲁肽对高脂诱导肥胖小鼠瘦素、脂肪分布及含量的影响 \=-had lower serum lipid level; the triglycerides level was significantly lower than that in the normal chow diet group and high fat diet group(P<0.05); and the total cholesterol level was significantly lower than that in high fat diet group(P<0.05). The liraglutide group was different from the other groups in the mesenteric, epididymal, perirenal, subscapular and subcutaneous fat(P<0.05). Conclusion Liraglutide can significantly reduce the body weight and leptin degree in obese mice and has an effect on regulating lipid and fat distribution.

Key words: Magnetic resonance imaging, Obesity, High fat diet, Liraglutide, Fat content, Leptin