JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (8): 17-21.doi: 10.6040/j.issn.1671-7554.0.2015.1031

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Effect of local slow-release coated sirolmus on fistula neointimal hyperplasia

NIU Jiyuan1, WANG Fangli2, ZHANG Ruibin3, GAO Qingzhen3, YUN Ping3, WANG Qi3, ZHANG Shan3, BAI Xue4, WANG Xiaoping3   

  1. 1. Taishan Medical University, Taian 271016, Shandong, China;
    2. Department of Nephrology, Pingyuan First Peoples Hospital, Dezhou 253100, Shandong, China;
    3. Kidney Disease Unit &
    Dialysis Department, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, Shandong, China;
    4. School of Medicine, Shandong University, Jinan 250012, Shandong, China
  • Received:2015-10-30 Online:2016-08-10 Published:2016-08-10

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Abstract: Objective To establish a model of cimino-brescia arteriovenous fistula by anastomosing rabbit carotid artery with internal jugular vein and to explore the mechanism of coated sirolmus in inhibiting the fistula anastomotic intimal hyperplasia. Methods A total of 18 healthy New Zealand male rabbits were randomly divided into blank control group(the grafts accepted no management), F-127 group(0.5 mL of 20% pluronic F-127 gel was locally applied on adventitia and anastomosis site)and F-127+sirolmus group(0.5 mL 20% pluronic F-127 gel containing 0.5 mg sirolmus was locally applied on adventitia and anastomosis site)to establish models of cimino-brescia arteriovenous fistula by anastomosing rabbit carotid artery with internal jugular vein. Three weeks after operation, the anastomotic veins were 山 东 大 学 学 报 (医 学 版)54卷8期 -牛纪媛,等.局部外膜缓释西罗莫司对内瘘血管内膜增生的影响 \=-harvested. The vein intimal hyperplasis was observed with tissue morphology method, the vascular intima thickness was measured with computer image system, and expressions of TGF-β1 and CTGF were detected with immunohistochemistry and western blotting. Results Three weeks after operation, the intima in the blank control group and F-127 group thickened obviously as compared with that in F-127+sirolmus group(P<0.05). The TGF-β1 and CTGF expression were higher in the blank control group and F-127 group than in the F-127+sirolmus group(P<0.05). There were no significant differences in the above parameters between the blank control group and F-127 group(P>0.05). Conclusion Sirolmus partial paintcoated in vascular adventitia inhibits the fistula neointimal hyperplasia via inhibition of TGF-β1 and CTGF expressions.

Key words: Cimino-brescia arteriovenous fistula, Intimal hyperplasia, Sirolmus, Transforming growth factor-β1, Connective tissue growth factor

CLC Number: 

  • R318.16
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