JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (4): 1-5.doi: 10.6040/j.issn.1671-7554.0.2015.1097

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Role of liraglutide in the repair of spinal cord injury

LI Hongzhi1,2, LIU Jing3, SONG Yan1,2, CHI Lingyi1,2, LIU Yuguang1,2   

  1. 1. Department of Neurosurgery, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China;
    2. Brain Hospital of Shandong University, Jinan 250012, Shandong, China;
    3. Department of Anesthesia, Taian Central Hospital, Taian 271000, Shandong, China
  • Received:2015-11-26 Online:2016-04-10 Published:2016-04-10

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Abstract: Objective To investigate the role and mechanism of liraglutide in the repair of spinal cord injury(SCI)in rats. Methods A total of 48 rat models of clip compressive SCI were established and then divided into 2 groups: liraglutide treatment group(n=24)and normal control group(n=24), which were injected with liraglutide(0.12 mg/kg, 0.5 mL)and physiological saline(0.5 mL)respectively after the operation. The apoptosis of neurocytes was detected with TUNEL method on day 2 after operation, the expressions of interleukin-1β and interleukin-6 were detected with Western blotting protein imprinting technology on day 3, the structure of injured spinal cord tissue was observed with HE staining on day 14, and the BBB score of hind limbs of rats behavior were recorded from day 3 to day 28. Results The rate of cell apoptosis, expression of inflammatory factors, and cavity area of the liraglutide treatment group were lower than those of the control group(all P<0.05), while the BBB scores of both hind limbs were higher than those in the control group at the same period(P<0.05). Conclusion Liraglutide treatment after spinal cord injury may reduce cell apoptosis acute inflammation, and is conducive to the late repair of nerve cells and the recovery of motor function.

Key words: Spinal cord injury, Liraglutide, Cell apoptosis, Interleukin-1β, Interleukin- 6, Inflammation, Rats

CLC Number: 

  • R574
[1] Poon P, Gupta DM, Tator C. Clip compression model is useful for thoracic spinal cord injuries:histologic and functional correlates[J]. Spine, 2007, 32(25):2853-2859.
[2] Rowland JW, Hawryluk GWJ, Brian K, et al. Current status of acute spinal cord injury pathophysiology and emerging therapies:promise on the horizon[J]. Neurosur Focus, 2008, 25(5):2.
[3] 王旭阳, 江敦清, 陈世文. 封闭内源性NT-3在脊髓损伤后期神经元有髓神经纤维出芽中的作用[J]. 山东大学学报(医学版), 2014, 52(12):5-9. WANG Xuyang, JIANG Dunqing, CHEN Shiwen. Function of endogenous NT-3 in the regeneration sprouting of large neuron at late-stage spinal cord injury[J]. Journal of Shandong University(Health Science), 2014, 52(12):5-9.
[4] Wang Q, Brubaker PL. Glucagon-like peptide-1 treatment delays the onset of diabetes in 8 week-old db/db mice[J]. Diabetologia, 2002, 45(9):1263-1273.
[5] Merchenthaler I, Lane M, Shughrue P. Distribution of pre-pro-glucagon and glucagon-like peptide-1 receptor messenger RNAs in the rat central nervous system[J]. J Comp Neurol, 1999, 403(2):261-280.
[6] Rachmany L, Tweedie D, Li Y, et al. Exendin-4 induced glucagon-like peptide-1 receptor activation reverses behavioral impairments of mild traumatic brain injury in mice[J]. Age(Dordr), 2013, 35(5):1621-1636.
[7] Ma T, Du X, Pick JE. Glucagon-like peptide-1 cleavage product GLP-1(9-36)amide rescues synaptic plasticity and memory deficits in Alzheimers disease model mice[J]. J Neurosci, 2012, 32(40):13701-13708.
[8] Holscher C. Central effects of GLP-1:new opportunities for treatments of neurodegenerative diseases[J]. J Endocrinol, 2014, 221(1):31-41.
[9] Cristina H, Marta GR, Lidia C, et al. Topical administration of somatostatin prevents retinal neurodegeneration in experimental diabetes[J]. Diabetes, 2013, 62(10):2569-2578.
[10] Sato K, Kameda M, Yasuhara T, et al. Neuroprotective effects of liraglutide for stroke model of rats[J]. Int J Mol Sci, 2013, 14(11):21513-21524.
[11] Zhang X, Li W, Li P, et al. Intraportal infusion of ghrelin could inhibit glucose-stimulated GLP-1 secretion by enteric neural net in Wistar rat[J]. Biomed Res Int, 2014, 2014(2):923564.
[12] Fleming J, Norenberg M, Ramsay D, et al. The cellular inflammatory response in human spinal cords after injury[J]. Brain, 2006, 129(Pt 12):3249-3269.
[13] Krasner NM, Ido Y, Ruderman NB. Glucagon-like peptide-1(GLP-1)analog liraglutide inhibits endothelial cell inflammation through a calcium and AMPK dependent mechanism[J]. PLoS One, 2014, 9(5):97554. doi:10.1371/journal.pone.0097554.eCollection 2014.
[14] McGovern SF, Hunter K, Holscher C. Effects of the glucagon-like polypeptide-1 analogue(Val8)GLP-1 on learning, progenitor cell proliferation and neurogenesis in the C57B/16 mouse brain[J]. Brain Res, 2012, 1473(6):204-213.
[15] Jolivalt CG, Fineman M, Deacon CF. GLP-1 signals via ERK in peripheral nerve and prevents nerve dysfunction in diabetic mice[J]. Diabetes Obes Metab, 2011, 13(11):990-1000.
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