JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (4): 6-10.doi: 10.6040/j.issn.1671-7554.0.2015.1000

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Effect of X-ray on the activity of MMP-2 and Cho/Cr ratio of U87 glioma multicellular spheroid

XU Yanjie1, CUI Yi1, LI Hongxia1, SHI Wenqi1, LI Fuyan1, WANG Jianzhen2, ZENG Qingshi1   

  1. 1. Department of Radiology;
    2. Department of Radiotherapy, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Received:2015-10-21 Online:2016-04-10 Published:2016-04-10

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Abstract: Objective To establish the U87 glioma multicellular spheroid(MCS)model and to explore the effect of various dosages of X-ray on the activity of MMP-2 and water-soluble metabolites of U87 glioma multicellular spheroids. Methods The U87 glioma MCS model was established and irradiated by X-ray of 0, 1, 5 and 10 Gy. The 0 Gg group served as the control group. Perchloric acid extracting(PCA)method was used to extract water-soluble metabolites, including choline(Cho), creatine(Cr), and N-acetylaspartate(NAA), which were observed using in vitro 14.7T high resolution 1H-MRS. The MMP-2 expression in U87 MCS model was detected with gelatin zymography. Results U87 glioma MCS model was established successfully. With the increase of X-ray doses, the Cho/Cr ratio rose from 2.74±0.02(1 Gy), 4.32±0.17(5 Gy)to 4.92±0.07(10 Gy); compared with the control group(2.10±0.29), the Cho/Cr ratio increased in the other groups［P=0.033(1 Gy), P=0.000(5 Gy), P=0.000(10 Gy)］. Compared with the control group, the activity of MMP-2 also increased(P=0.000). Conclusion Sublethal irradiation promotes the activity of MMP-2 of U87 MCS and elevates the Cho/Cr ratio.

Key words: Glioma, Multicellular spheroid, Proton magnetic resonance spectroscopy, Matrix metalloproteinase 2, Cho/Cr

CLC Number: 

  • R445.2
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