JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (9): 8-12.doi: 10.6040/j.issn.1671-7554.0.2015.219

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Effects of factors secreted by mouse embryonic stem cells on prostate cancer cells in vitro

ZHAI Hongyun1, ZHANG Denglu1, WANG Guangjie1, KONG Feng2, CHENG Guanghui2, ZHAO Shengtian1   

  1. 1. Department of Urology, Jinan 250033, Shandong, China;
    2. Central Laboratory, Second Hospital of Shandong University, Jinan 250033, Shandong, China
  • Received:2015-02-28 Online:2015-09-10 Published:2015-09-10
  • Contact: 赵升田。E-mail:zhaoshengtian@sdu.edu.cn E-mail:zhaoshengtian@sdu.edu.cn

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Abstract: Objective To study the effects of factors secreted by mouse embryonic stem cells (ESC) on the biological characteristics of prostate cancer cells RM-1 and to explore the mechanism. Methods A non-contact co-culture system was established with Transwell chambers in vitro. The chambers connecting ESC combined with mouse embryonic fibroblasts (MEF) and RM-1 cells served as experimental group, while the chambers connecting MEF and RM-1 cells were negative controls. After cells were co-cultured for 72 h, the proliferation of RM-1 cells in the two groups were measured. Differences of RM-1 cells in cell morphology, cell cycle, apoptotic level, migration and invasion ability between the two groups were observed. Results The RM-1 cells in experimental group were loosely arranged with less cell division and more cell death, and some cells showed signs of apoptosis. The RM-1 cells in experimental group proliferated more slowly (P<0.01), with cell cycle arrest between phase G1 and S in addition with increased apoptosis, especially late stage apoptosis (both P<0.01). The RM-1 cells in experimental group showed weaker migration and invasion ability (both P<0.05). Conclusion The factors secreted by ESC exhibited anti-tumor effects. They inhibited RM-1 cell proliferation by arresting cell cycle, increasing cell apoptosis, and weakening cell migration and invasion ability.

Key words: Secreted factors, Prostate cancer cell RM-1, Cellular environment, Embryonic stem cell, Mouse, Neoplasia

CLC Number: 

  • R737.25
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