JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (9): 13-18.doi: 10.6040/j.issn.1671-7554.0.2015.281

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Effects of nitidine chloride on the proliferation and apoptosis of prostate cancer PC-3 cells

CHENG Xiangyu1, XING Rui2, XING Zhaoquan1, GUO Zhaoxin1, GUO Xiaoyu3, SU Jing3, MENG Liwei1, LIU Zhaoxu1,3   

  1. 1. Department of Urology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China;
    2. Department of Urology, Peoples Hospital of Linyi, Linyi 251500, Shandong, China;
    3. School of Nursing, Shandong University, Jinan 250012, Shandong, China
  • Received:2015-03-16 Online:2015-09-10 Published:2015-09-10
  • Contact: 刘照旭。E-mail:zhaoxvl@sdu.edu.cn E-mail:zhaoxvl@sdu.edu.cn

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Abstract: Objective To evaluate the effects of nitidine chloride on the proliferation and apoptosis of prostate cancer cells PC-3 in vitro. Methods MTT, scratch migration and Transwell were used to examine the changes in the proliferation and invasions of human prostate cancer cell PC-3 after nitidine chloride treatment. Flow cytometry was used to examine the changes in cell apoptosis of PC-3. Immunoblot analysis was adopted to detect the expression of AKT/mTOR, Bcell-associated x protein (Bax) and Bcell lymphoma-2 (Bcl-2). A specific PI3K inhibitor, LY294002, was used to evaluate the influence of prostate cancer cells by inhibiting AKT pathway. Results Nitidine chloride inhibited the proliferation and invasion of PC-3 cells in a dose-dependent manner (P<0.01). Nitidine chloride inhibited AKT and mTOR pathways phosphorylation, accompanied by up-regulation of Bax, and down-regulation of Bcl-2. Nitidine chloride significantly increased the ratio of Bax/Bcl-2 (P<0.01), to induce prostate cancer cells apoptosis. Furthermore, combined use of LY294002 inhibited AKT pathway, which could enhance the anti-proliferation and anti-invasion effectsof nitidine chloride. Conclusion Nitidine chloride can suppress the proliferation, migration, invasion and induce the apoptosis of prostate cancer cells in vitro. Nitidine chloride also plays a role in anti-cancer via inhibiting AKT/mTOR pathway phosphorylation, which implies that nitidine chloride may be a promising therapeutic drug for prostate cancer.

Key words: Prostate neoplams, Apoptosis, Nitidine chloride, AKT /mTOR pathway, Cell metastasis

CLC Number: 

  • R737.25
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