Abstract:

Objective   To study the anti-tumor effect of recombinant adeno-associated virus mediated secretive expression of NT4-TAT-6×His-VHLβ fusion peptide (rAAV/NT4-TAT-6×His-VHLβ) on the chorioallantoic membrane(CAM) implanted tumors. Methods   RLC-310 cells were inoculated on the CAM . Chick embryos with implanted tumor were divided into three groups, and respectively treated with rAAV/NT4-TAT-6×His-VHLβ(group A), AAV (group B) and PBS(group C). The growth of implanted tumors were observed, and the size of tumors on fifth day after the treatment was measured. Then the effect of anti-tumor in each group was observed through HE dye and immunofluorescence. Results   The results showed that the growth of implanted tumors in group A was slower than the other two groups. The tumor volumes of the group A showed significant difference compared with group B and C (P<0.05). The growth ability of tumors was inhibited in group A. Bright fluorescent appeared in the group A(++). No fluorescence was found in the contrast group (-). Conclusion   rAAV/NT4-TAT-6×His-VHLβ has the anti-tumor effect on chick embryo implanted tumors, which may be a novel tool for gene therapy of human renal cell carcinoma.

Key words: Kidney neoplasms; Chick embryo chorioallantoic membrane; Fusion peptide; Gene therapy; Animal model