Abstract:

Objective   To study the effect of Jianpiyishen-Huoxuehuayu Chinese herbal compound prescription on renal mesangial cell proliferation and expressions of TGF-β1 and BMP-7 in high glucose. Methods   RMCs of conventional culture were divided into the normal control group, mannitol group, high glucose group and high glucose with 0.65mg/mL traditional Chinese medicine group. The glucose concentration of normal group was 5.6mmol/L while 30mmol/L in the high glucose group. After 48h, cell proliferation was detected with 4 -A thiazolyl blue. TGF-β1 was detected with ELISA. BMP-7 mRNA was detected with RT-PCR. Results   Compared with the blank control group, cell proliferation and expression of TGF-β1 of high glucose group were obviously increased while the level of BMP-7 mRNA was decreased. Compared with the high glucose group, cell proliferation and expression of TGF-β1 of Chinese herbal compound prescription obviously decreased while expression of BMP-7 mRNA was increased. Conclusion   High glucose could promote the proliferation and expression of TGF-β1 of RMC. It also could decrease the expression of BMP-7 mRNA. Chinese herbal compound prescription could restrain the proliferation and expression of TGF-β1 of RMC in high glucose while it could increase the expression of BMP-7 mRNA.

Key words: Rat mesangial cells; Jianpiyishen-Huoxuehuayu Chinese herbal compound prescription; Tissue growth factor-β1; Bone morphogenetic protein-7