Abstract:

Objective〓〖WTBZ〗To explore the protective effect of butyphthalide on hippocampus neurons in epileptic rats. 〖WTHZ〗Methods〓〖WTBZ〗30 adult male Wistar rats were randomly divided into three groups: the intervention group, the epilepsy group and the control group. The intervention group was given 80mg/Kg butyphthalide via i.p injection and the epilepsy group was given 80mg/Kg 0.9%NaCl the same way. Rats were killed 3 or 24hours after the end of lesion, and then the hippocampus was taken out and observed by light microscopy and electron microscopy and the levels of superoxide dismutase(SOD) and malonadialdehyde(MDA) were determined. 〖WTHZ〗Results〓〖WTBZ〗The epileptic paroxysm latent period of the intervention group was significantly prolonged, the results of HE staining and electron microscopy showed that there was less cellular swelling and plasmatorrhexis, and there were few neurons in the intervention group. Bioblasts were deformed, crista mitochondriales disappeared, and cavitation and membranous bodies were found in the kytoplasm. In the butyphthalide intervention group, cellular construction was roughly normal, but the nucleoli were collected aside, and the construction of the chondriosomes was still integrated. There were few membranous bodies, but the membrane broke and cristae mitochondriale depletion still appeared in some of the neurons. Zymology showed butyphthalide can raise the activation of SOD(P<0.01，P<0.05) and decrease the quantity of MDA(P<0.05, P<0.01). 〖WTHZ〗
Conclusion〓〖WTBZ〗Butyphthalide can decrease hippocampus neuron damage caused by temporal epilepsy, which is related to the protective effect of butyphthalide on chondrosomes.

Key words: Status epilepticus, Ultrastructure, Superoxide dismutase, Malondialdehyde, Butyphthalide, Rats, inbred strains