JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)

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Simvastatin promotes the proliferation and mineralization of osteoblasts cultured in vitro

YANG Yun1，ZHANG Li-ping1，HU Jian-li1，ZHANG Zuo-lun2   

  1. 1.School of Medicine, Shandong University；2.Shangdong Provincial Hospital
  • Received:2007-01-22 Revised:1900-01-01 Online:2007-09-24 Published:2007-09-24
  • Contact: YANG Yun

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Abstract: Objective: To study the effect of Simvastatin on the proliferation and mineralization of cultured rat calvaria osteoblasts (RCOBs). Methods: RCOBs were cultured in vitro by an enzyme-digestion method and treated with Simvastatin and rhBMP-2 in series gradient of concentration for 24 hours. Then the viability and proliferation of RCOBs were determined by MTT assay. After implantation in a 12well microtiter plate for 7 days, the mineralization nodes were stained by the von Kossa method. Photos were transferred into a black-white style and the mean mineralization area ratio was measured. Results: Simvastatin could not only stimulate the proliferation of the RCOBs in a dosedependent manner， but also increased the mineralization ratio compared with the control group, which mimicked rhBMP-2. Conclusion: Simvastatin can promote the proliferation and mineralization of RCOBs in vitro, which may partially be attributed to the osteogenesis mechanism of statins.

Key words: Simvastatin, Osteoblast, MTT assay, Proliferation, Mineralization

CLC Number: 

  • R681.3
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