JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)

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bFGF and bcl-2 in guinea pig's sclera with lens-induced myopia

WANG Hong1，TAO Yuan1，QU Yi1，WANG Zhen-hong2   

  1. Department of Opthalmology, 1.Qilu Hospital of Shandong University, Jinan 250012；2.Jimo City Hospital of Traditional Chinese
  • Received:2007-01-03 Revised:1900-01-01 Online:2007-05-24 Published:2007-05-24
  • Contact: QU Yi

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Abstract: Objective：To explore the expression of B-cell lymphoma/leukemia 2 and basic fibroblast growth factor in lens-induced myopia sclera of guinea pigs and evaluate the effect of the two factors on apoptosis of the sclera cells. Methods: A -20D concave or -10D concave lens was worn over the right eye in 30 fourweek old pigmented guinea pigs. The eyes were periodically examined by retinoscopy, and A-scan ultrasonography. Two weeks later, the eyeballs were removed and the apoptotic cells were determined by electron microscopy, TUNEL technique and flow cytometry in scleras. Both bFGF and the bcl-2 protein were determined by the immunohistochemisty method and the Western-blot method. Results: All eyes treated with -10.00D or -20.00D concave lens produced defocus-induced myopia. Axial lengths in lensinduced eyes remarkably increased compared with control eyes. Apoptotic cells were observed in fibroblasts of sclera in lens-induced eyes, much more than in control eyes (P＜0.01). Expression of the bFGF and bcl-2 protein was decreased (P＜0.01). With an increase of the diopter in the lens-induced myopia of guinea pigs, expression of bFGF and bcl-2 was decreased. Conclusion: bFGF and bcl-2 may play critical roles in guinea pig′s sclera with lens-induced myopia by adjustment of the apoptosis.

Key words: Myopia, Apoptosis, Fibroblast growth factor2, B-cell lymphoma/leukemia 2, Sclera

CLC Number: 

  • R778.11
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