JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)

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Role of the SPK/S1P signal in the expression of VEGF in human hepatoma cells

JI Da-lei1，GAO Yan-jing1，FU Jin-dong1，SONG Jian-hua2，XU Jjian-min1   

  1. 1. Department of Gartroenterology, Qilu Hospital of Shandong University；2. Department of Radiation, Affiliated Hospital of Weifang Medical College
  • Received:2006-11-28 Revised:1900-01-01 Online:2007-03-24 Published:2007-03-24
  • Contact: GAO Yan-jing

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Abstract: Objective：To investigate the role of the SPK/S1P（sphingosine kinase/sphingosine-1-phospate） signal in regulating the expression of the vascular endothelial growth factor(VEGF) in human hepatoma cells. Methods: The human hepatoma cell line HepG2 was cultured in vitro. After treatment with a concentration of 5-20μmol/L DMS (dimethyl sphingosine) in culture medium，the VEGF protein levels at 12h were measured by an enzyme-linked immunosorbent assay(ELISA). Results: The expression of VEGF was obviously inhibited by DMS in a dose-dependent manner. Conclusion: DMS makes the VEGF expression decrease by inhibiting the SPK/S1P signal, suggesting that the SPK/S1P signal regulates the expression of VEGF in hepatoma cells.

Key words: Signal transduction, Carcinoma, hepatocellular, Endothelium, vascular, Growth substances

CLC Number: 

  • R735.7
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