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山东大学学报 (医学版) ›› 2020, Vol. 58 ›› Issue (3): 94-98.doi: 10.6040/j.issn.1671-7554.0.2019.1405

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MiR-122与miR-33a在2型糖尿病合并冠心病患者中的表达

金海燕1,张炎1,2,马小莉1,韩羽1,3,赵蕙琛1,刘元涛1,张玉超1   

  1. 1. 青岛市市立医院内分泌科, 山东 青岛 266011;2. 赤峰市医院内分泌科, 内蒙古自治区 赤峰 024000;3. 南阳市第二人民医院, 河南 南阳 473000
  • 出版日期:2020-03-10 发布日期:2022-09-27
  • 通讯作者: 张玉超. E-mail:yc.zhang@hotmail.com

Expressions of miR-122 and miR-33a in patients with type 2 diabetes complicated with coronary artery disease

JIN Haiyan1, ZHANG Yan1,2, MA Xiaoli1, HAN Yu1,3, ZHAO Huichen1, LIU Yuantao1, ZHANG Yuchao1   

  1. 1. Department of Endocrinology, Qingdao Municipal Hospital, Qingdao 266011, Shandong, China;
    2. Department of Endocrinology, Chifeng Municipal Hospital, Chifeng 024000, Inner Mongolia, China;
    3. Nanyang Second General Hospital, Nanyang 473000, Henan, China
  • Online:2020-03-10 Published:2022-09-27

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摘要： 目的 探讨2型糖尿病(T2DM)合并冠状动脉粥样硬化性心脏病(CAD)患者血清miR-122和miR-33a表达的改变及其意义。 方法 收集2016年7月至2018年7月收治的2型糖尿病患者32例(T2DM组)、冠心病患者32例(CAD组)、2型糖尿病合并冠心病患者32例(T2DM+CAD组)的临床资料,另选择同期体检的健康者32例(对照组)。经分离提纯血浆总microRNA,采用实时定量PCR法检测miR122和miR33a的表达量。 结果 与对照组相比,T2DM组、CAD组和T2DM+CAD组血浆miR122mRNA表达量明显增加(P<0.05);与T2DM组相比,CAD组和T2DM+CAD组miR-122mRNA表达量明显增加(P<0.05)。miR-122对CAD组和T2DM+CAD组的ROC曲线下面积分别为0.72±0.05与0.77±0.04。各组间miR-33a表达水平无明显差异(P>0.05)。 结论 血浆miR-122是2型糖尿病合并冠心病较好的辅助诊断指标。

关键词: miR122, miR33a, 2型糖尿病, 冠状动脉粥样硬化性心脏病

Abstract: Objective To investigate the expressions and diagnostic significance of serum miR-122 and miR-33a in patients with type 2 diabetes mellitus(T2DM)complicated with coronary artery heart disease(CAD). Methods Fast bloods samples were collected from 32 healthy volunteers(NC group), 32 T2DM patients(T2DM group), 32 CAD patients(CAD group)and 32 patients with T2DM and CAD(T2DM+CAD group). The expressions of miR-122 and miR-33a were detected with real-time quantitative PCR. Specificity and sensitivity of the association between miR-122 in T2DM and CAD were evaluated with receiver operating characteristic(ROC)and area under the curve(AUC). Results Compared with the NC group, the other three groups had significantly higher expressions of miR-122(P<0.05). Compared with T2DM group, the CAD and T2DM+CAD groups had significantly higher expression of miR-122(P<0.05). The AUC of miR-122 in CAD group and T2DM+CAD group was 0.72±0.05 and 0.77±0.04, respectively. There were no significant differences in the miR-33a expression among the four groups(P>0.05). Conclusion Serum miR-122 can serve as a good diagnostic marker for T2DM complicated with CAD.

Key words: miR-122, miR-33a, Type 2 diabetes mellitus, Coronary artery heart disease

中图分类号: 

  • R574
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