2型糖尿病患者外周血突触融合蛋白8表达与慢性炎症、糖脂代谢的相关性

doi:10.6040/j.issn.1671-7554.0.2018.786

摘要/Abstract

摘要： 目的探讨2型糖尿病(T2DM)患者外周血突触融合蛋白8(STX8)表达与慢性炎症、糖脂代谢的相关性。方法选择144例初诊T2DM患者(病例组)与100例体检者(对照组)作为研究对象,比较两组一般资料、炎症指标、糖脂代谢指标的差异。分离两组患者外周血单核细胞(PBMCs),比较两组PBMCs细胞中STX8表达。分别用标准葡萄糖组(10 mmol/L)和高糖组(33.3 mmol/L)处理对照组PBMCs,检测不同处理组PBMCs细胞STX8表达和炎症因子水平。结果病例组体质量指数(BMI)、空腹血糖(FBG)、空腹血浆胰岛素(FIN)、总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、游离脂肪酸(FFAs)、胰岛素抵抗指数(HOMA-IR)、肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)、STX8水平明显高于对照组,HDL-C明显低于对照组,差异有统计学意义(P均<0.05)。病例组PBMCs细胞STX8 mRNA和蛋白表达明显高于对照组,差异有统计学意义(P均<0.05)。病例组患者外周血STX8浓度与FBG、FIN、TC、TG、LDL-C、FFAs、HOMA-IR、TNF-α、IL-6呈正相关,与HDL-C呈负相关(P均<0.05)。高糖处理后,PBMCs细胞STX8蛋白表达和TNF-α、IL-6含量明显增加;高糖组各时间点(6、24、48、72 h)STX8蛋白表达和TNF-α、IL-6含量明显高于标准葡萄糖组,差异有统计学意义(P均<0.05)。结论 T2DM患者外周血STX8表达与慢性炎症、糖脂代谢异常有关。

关键词: 2型糖尿病, 突触融合蛋白8, 炎症反应, 糖脂代谢

Abstract: Objective To investigate Syntaxin 8(STX8)expression in patients with type 2 diabetes mellitus(T2DM)and the potential correlations between STX8 and chronic inflammation and glucose/lipid metabolism. Methods A total of 144 T2DM paitents(case group)and 100 health subjects(control group)were enrolled. The difference of clinical parameters such as inflammatory indices and glucose/lipid metabolism were compared between two groups. Meanwhile, peripheral blood mononuclear cells(PBMCs)were collected and their expressions were compared in these two groups. PBMCs isolated from healthy subjects were cultured with 10 mmol/L glucose or 33.3 mmol/l glucose, respectively. The levels of STX8 expression and inflammatory cytokines were detected. Results The levels of body mass index(BMI), fasting blood glucose(FBG), fasting plasma insulin(FIN), total cholesterol(TC), trilycerides(TG), low-density lipoprotein cholesterol(LDL-C), free fatty acids(FFAs), homeostasis model assessment insulin resistence(HOMA-IR), tumor necrosis factor-α(TNF-α), interleukin 6(IL-6), STX8 in case group were significantly higher than that in control group, while HDL-C level in case group was significantly lower than that in control group(all P<0.05). The mRNA and protein expressions of STX8 in PBMCs of case group were significantly higher compared with that in control group(P<0.05). STX8 expression showed a positive correlation with FBG, FIN, TC, TG, LDL-C, FFAs, HOMA-IR, 山东大学学报 (医学版)56卷12期 -陈攀,等.2型糖尿病患者外周血突触融合蛋白8表达与慢性炎症、糖脂代谢的相关性 \=-TNF-α, IL-6, while a negative correlation with HDL-C(all P<0.05). After treatment with high concentration, the levels of STX8 and TNF-α, IL-6 significantly increased, STX8 expression and TNF-α, IL-6 concentrations in 33.3 mmol/L glucose group was markedly higher than that in 10 mmol/L glucose group(all P<0.05). Conclusion STX8 level may be correlated with chronic inflammatory state and perturbed glucose and lipid metabolism in T2DM patients.

Key words: Type 2 diabetes mellitus, Syntaxin 8, Inflammation, Glucose and lipid metabolism

中图分类号:

R587.1

陈攀,蒋春霞,雷艺. 2型糖尿病患者外周血突触融合蛋白8表达与慢性炎症、糖脂代谢的相关性[J]. 山东大学学报 (医学版), 2018, 56(12): 26-32.

CHEN Pan, JIANG Chunxia, LEI Yi. Correlation between peripheral Syntaxin 8 expression and chronic inflammation and glucose-lipid metabolism in type 2 diabetes mellitus patients[J]. Journal of Shandong University (Health Sciences), 2018, 56(12): 26-32.

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